MUC1粘蛋白单次重复序列的MHC限制性呈递。

MHC-restricted presentation of a single repeat of MUC1 mucin.

作者信息

Orr Melody K, Burnside Janet S, Phillips Catherine A, Philip Ramila, Dombrowski Kenneth E, Wright Stephen E

机构信息

Department of Internal Medicine and Women's Health and Research Institute, Texas Tech University Health Sciences Center, Amarillo, Texas 79106, USA.

出版信息

Immunol Invest. 2007;36(3):271-83. doi: 10.1080/08820130601187384.

DOI:10.1080/08820130601187384

PMID:17558710

Abstract

Major histocompatibility complex (MHC) restriction of antigen presentation of a single mucin1 (MUC1) variable number of tandem repeats peptide (VNTR1) was examined by generating cytotoxic T lymphocytes (CTL) derived from peripheral blood mononuclear cells (PBMC) stimulated with a single repeat MUC1 peptide presented by allogeneic (MHC-independent) or autologous (MHC-dependent) Epstein-Barr Virus (EBV) immortalized B lymphocytes. The ability to generate greater CTL activity against MUC1-expressing tumor cells by stimulation with autologous versus allogeneic EBV-B supports the hypothesis that presentation of a single repeat of MUC1 peptide is MHC-restricted (MHC-dependent).

摘要

通过用同种异体（不依赖主要组织相容性复合体，MHC）或自体（依赖MHC）的爱泼斯坦-巴尔病毒（EBV）永生化B淋巴细胞呈递的单个重复黏蛋白1（MUC1）串联重复肽（VNTR1）刺激外周血单个核细胞（PBMC）产生细胞毒性T淋巴细胞（CTL），来检测单一MUC1 VNTR1肽抗原呈递的MHC限制性。与同种异体EBV - B刺激相比，自体EBV - B刺激产生更强的针对表达MUC1肿瘤细胞的CTL活性，这支持了单一重复MUC1肽的呈递受MHC限制（依赖MHC）这一假说。