Kang Wonku, Jeong Ji-Hoon, Ma Eunsook, Kwon Kwang-il
College of Pharmacy, Catholic University of Daegu, Kyoungbuk 712-702, South Korea.
J Pharm Biomed Anal. 2007 Sep 3;44(5):1178-82. doi: 10.1016/j.jpba.2007.04.028. Epub 2007 Apr 29.
A simple and rapid quantification method was developed for determining menatetrenone and its epoxide metabolite in human plasma. After a simple protein precipitation with methanol, the analytes were chromatographed on a reversed-phase C(18) column and detected by LC/MS/MS with atmospheric pressure chemical ionization. The coefficient of variation of the assay precision was less than 10.1%, and the accuracy ranged from 98.0 to 106.5%. The limit of detection of menatetrenone and its epoxide was 0.5 and 0.2 ng/ml, respectively. This method was used to simultaneously measure the plasma concentration of menatetrenone and its epoxide metabolite from healthy subjects after a single 30 mg oral dose of menatetrenone.
开发了一种简单快速的定量方法，用于测定人血浆中维生素K2及其环氧化物代谢物。用甲醇进行简单的蛋白质沉淀后，将分析物在反相C(18)柱上进行色谱分离，并用大气压化学电离的液相色谱-串联质谱法进行检测。该测定精密度的变异系数小于10.1%，准确度范围为98.0%至106.5%。维生素K2及其环氧化物的检测限分别为0.5和0.2 ng/ml。该方法用于在单次口服30 mg维生素K2后，同时测量健康受试者血浆中维生素K2及其环氧化物代谢物的浓度。
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