耐甲氧西林金黄色葡萄球菌Sar2028（一种天冬氨酸/酪氨酸/苯丙氨酸磷酸吡哆醛依赖性转氨酶）的表达、纯化、结晶、数据收集及初步生化特性分析
Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase.
作者信息
Seetharamappa Jaldappagari, Oke Muse, Liu Huanting, McMahon Stephen A, Johnson Kenneth A, Carter Lester, Dorward Mark, Zawadzki Michal, Overton Ian M, van Niekirk C A Johannes, Graham Shirley, Botting Catherine H, Taylor Garry L, White Malcolm F, Barton Geoffrey J, Coote Peter J, Naismith James H
机构信息
Scottish Structural Facility and Centre for Biomolecular Sciences, The University, St Andrews KY16 9ST, Scotland.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):452-6. doi: 10.1107/S1744309107019562. Epub 2007 Apr 28.
Abstract
Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase with a molecular weight of 48,168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified and crystallized. The protein crystallized in a primitive orthorhombic Laue group with unit-cell parameters a = 83.6, b = 91.3, c = 106.0 A, alpha = beta = gamma = 90 degrees. Analysis of the systematic absences along the three principal axes indicated the space group to be P2(1)2(1)2(1). A complete data set was collected to 2.5 A resolution.
摘要
Sar2028是一种天冬氨酸/酪氨酸/苯丙氨酸磷酸吡哆醛依赖性转氨酶，分子量为48,168道尔顿，与甲氧西林敏感菌株相比，在耐甲氧西林金黄色葡萄球菌中过表达。该蛋白在大肠杆菌中表达、纯化并结晶。该蛋白在原始正交劳厄群中结晶，晶胞参数为a = 83.6、b = 91.3、c = 106.0 Å，α = β = γ = 90°。沿三个主轴的系统消光分析表明空间群为P2(1)2(1)2(1)。收集了分辨率为2.5 Å的完整数据集。
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