Lee Joung Wook, Namkoong Hong, Kim Hyun Kee, Kim Sanghee, Hwang Dong Whi, Na Hae Ri, Ha Seon-Ah, Kim Jae-Ryong, Kim Jin Woo
Department of Neurology, Bobath Memorial Hospital, Bundang-Gu, Seongnam, Gyungki-do, Korea.
BMC Neurol. 2007 Jun 12;7:14. doi: 10.1186/1471-2377-7-14.
Cerebrospinal fluid (CSF) may be valuable for exploring protein markers for the diagnosis of Alzheimer's disease (AD). The prospect of early detection and treatment, to slow progression, holds hope for aging populations with increased average lifespan. The aim of the present study was to investigate candidate CSF biological markers in patients with mild cognitive impairment (MCI) and AD and compare them with age-matched normal control subjects.
We applied proteomics approaches to analyze CSF samples derived from 27 patients with AD, 3 subjects with MCI and 30 controls. The AD group was subdivided into three groups by clinical severity according to clinical dementia rating (CDR), a well known clinical scale for dementia.
We demonstrated an elevated level of fibrinogen gamma-A chain precursor protein in CSF from patients with mild cognitive impairment and AD compared to the age-matched normal subjects. Moreover, its expression was more prominent in the AD group than in the MCI and correlated with disease severity and progression. In contrast, fibrinogen gamma-A chain precursor protein was detected very low in the age-matched normal group.
These findings suggest that the CSF level of fibrinogen gamma-A chain precursor may be a candidate biomarker for AD.
脑脊液（CSF）对于探索用于阿尔茨海默病（AD）诊断的蛋白质标志物可能具有重要价值。早期检测和治疗以减缓疾病进展，为平均寿命增加的老年人群带来了希望。本研究的目的是调查轻度认知障碍（MCI）和AD患者脑脊液中的候选生物学标志物，并将其与年龄匹配的正常对照受试者进行比较。
我们应用蛋白质组学方法分析了来自27例AD患者、3例MCI患者和30例对照的脑脊液样本。根据临床痴呆评定量表（CDR，一种著名的痴呆临床量表），将AD组按临床严重程度分为三组。
我们发现，与年龄匹配的正常受试者相比，轻度认知障碍和AD患者脑脊液中纤维蛋白原γ-A链前体蛋白水平升高。此外，其在AD组中的表达比在MCI组中更显著，且与疾病严重程度和进展相关。相比之下，在年龄匹配的正常组中检测到的纤维蛋白原γ-A链前体蛋白水平非常低。
这些发现表明，脑脊液中纤维蛋白原γ-A链前体水平可能是AD的候选生物标志物。
