[通过靶向基因转移抑制涎腺腺样囊性癌的增殖]
[Inhibition of proliferation of salivary adenoid cystic carcinoma by targeting gene transfer].
作者信息
Su Tao, Sun Hong-chen, Zang Guang-xiang, Zhang Hong, Lu Dong-min
机构信息
Department of Oral Pathology, School of Stomatology, Jilin University, Changchun 130041, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2007 Mar;42(3):184-5.
OBJECTIVE
To investigate the expression of gene TRAIL driven by human telomerase reverse transcriptase (hTERT) promoter in SACC-83 cell tumor necrosis factor related apoptosis in dncing ligand.
METHODS
After pACTERT-TRAIL plasmid transfected was into SACC-83 and HEL cells through liposome, the expression of TRAIL was examined using RT-PCR technique, the cells' survival rate by methyl thiazolyl tetrazolium (MTT) method and apoptosis rate by flow cytometry.
RESULTS
Expression of extrinsic TRAIL gene driven by hTERT promoter was detected in SACC-83 cells, and not detected in human embryonic lung fibroblast (HEL) cells. After transfection of pACTERT-TRAIL, the proliferation of SACC-83 cells was significantly inhibited, and its apoptotic rate was promoted, whereas no inhibited effect was observed on HEL cells and its apoptotic rate showed little change.
CONCLUSIONS
hTERT promoter can be used to induce tumor-specific expression of TRAIL gene and apoptosis in SACC-83 cells.
目的
研究人端粒酶逆转录酶（hTERT）启动子驱动的肿瘤坏死因子相关凋亡诱导配体（TRAIL）基因在SACC - 83细胞中的表达。
方法
通过脂质体将pACTERT - TRAIL质粒转染至SACC - 83细胞和人胚肺成纤维细胞（HEL）中，采用逆转录聚合酶链反应（RT - PCR）技术检测TRAIL的表达，用噻唑蓝（MTT）法检测细胞存活率，用流式细胞术检测细胞凋亡率。
结果
在SACC - 83细胞中检测到hTERT启动子驱动的外源性TRAIL基因表达，而在人胚肺成纤维细胞（HEL）中未检测到。转染pACTERT - TRAIL后，SACC - 83细胞的增殖受到显著抑制，凋亡率升高，而对HEL细胞无抑制作用，其凋亡率变化不大。
结论
hTERT启动子可用于诱导TRAIL基因在SACC - 83细胞中的肿瘤特异性表达并诱导其凋亡。
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