Ji David, Roman Mark, Zhou Joseph, Hildreth Jana
Analytical Laboratories in Anaheim, Inc., 2951 Saturn St, Unit C, Brea, CA 92821, USA.
J AOAC Int. 2007 May-Jun;90(3):659-69.
A method to quantify chondroitin sulfate in raw materials and dietary supplements at a range of about 5 to 100% (w/w) chondroitin sulfate has been developed and validated. The chondroitin sulfate is first selectively hydrolyzed by chondroitinase ACII enzyme to form un-, mono-, di-, and trisulfated unsaturated disaccharides; the resulting disaccharides are then quantified by ion-pairing liquid chromatography with ultraviolet detection. The amounts of the individual disaccharides are summed to yield the total amount of chondroitin sulfate in the material. Single-laboratory validation has been performed to determine the repeatability, accuracy, selectivity, limit of detection, limit of quantification, ruggedness, and linearity of the method. Repeatability precision for total chondroitin sulfate content was between 1.60 and 4.72% relative standard deviation, with HorRat values between 0.79 and 2.25. Chondroitin sulfate recovery from raw material negative control was between 101 and 102%, and recovery from finished product negative control was between 105 and 106%.
已开发并验证了一种在硫酸软骨素含量约为5%至100%（w/w）的范围内对原材料和膳食补充剂中的硫酸软骨素进行定量的方法。硫酸软骨素首先被硫酸软骨素酶ACII选择性水解，形成未硫酸化、单硫酸化、二硫酸化和三硫酸化的不饱和二糖；然后通过离子对液相色谱-紫外检测对生成的二糖进行定量。将各个二糖的量相加，得出材料中硫酸软骨素的总量。已进行单实验室验证，以确定该方法的重复性、准确性、选择性、检测限、定量限、耐用性和线性。硫酸软骨素总含量的重复性精密度相对标准偏差在1.60%至4.72%之间，HorRat值在0.79至2.25之间。原材料阴性对照中硫酸软骨素的回收率在101%至102%之间，成品阴性对照中硫酸软骨素的回收率在105%至106%之间。
