多核苷酸在硬脂酸朗缪尔-布洛杰特单分子层上吸附过程中的自组装效应。

Self-assembly effect during the adsorption of polynucleotides on stearic acid langmuir-blodgett monolayer.

作者信息

Dubrovin Evgeniy V, Staritsyn Sergey N, Yakovenko Sergey A, Yaminsky Igor V

机构信息

Department of Physics of Polymers and Crystals, Faculty of Physics, Moscow State University, Leninskie Gory, Moscow, Russia.

出版信息

Biomacromolecules. 2007 Jul;8(7):2258-61. doi: 10.1021/bm0702096. Epub 2007 Jun 21.

DOI:10.1021/bm0702096

PMID:17583947

Abstract

Interaction of polyadenylic acid, poly(A), with stearic acid Langmuir-Blodgett (LB) monolayer was studied in different electrolyte surroundings. For this purpose LB films of stearic acid, transferred on the mica substrate from poly(A) containing subphase, were analyzed with atomic force microscopy (AFM). The density of polynucleotides surface coverage is ruled by the monovalent electrolyte concentration in the subphase that is in good agreement with previous results. Divalent cations in the subphase are needed to stabilize poly(A) molecules on the surface through formation of "salt bridges". At the very low divalent electrolyte concentration polynucleotides adsorb on the LB film to domains in which the effect of self-assembly is observed. Increase of divalent electrolyte concentration leads to the loss of this orientation effect. The explanation of this effect is proposed.

摘要

研究了在不同电解质环境下聚腺苷酸（poly(A)）与硬脂酸朗缪尔-布洛杰特（LB）单分子层之间的相互作用。为此，使用原子力显微镜（AFM）分析了从含poly(A)的亚相转移到云母基底上的硬脂酸LB膜。多核苷酸表面覆盖密度受亚相中单价电解质浓度的控制，这与先前的结果高度一致。亚相中的二价阳离子通过形成“盐桥”来稳定表面的poly(A)分子。在极低的二价电解质浓度下，多核苷酸吸附到LB膜上形成可观察到自组装效应的区域。二价电解质浓度的增加导致这种取向效应的丧失。本文提出了对此效应的解释。