有心血管危险因素的菲律宾人群中人类胆固醇酯转运蛋白（TaqIB）多态性

Human cholestryl ester transfer protein (TaqIB) polymorphism among Filipinos with cardiovascular risk factors.

作者信息

Sy Rody G, Cutiongco Eva Maria C, Punzalan Felix Eduardo R, Santos Ronald S, Geronimo Francis Ruel B, Tangco Rogelio V

机构信息

Lipid Research Unit, Department of Medicine, University of the Philippines-Philippine General Hospital, Manila, Philippines.

出版信息

J Atheroscler Thromb. 2007 Jun;14(3):116-21. doi: 10.5551/jat.14.116.

DOI:10.5551/jat.14.116

PMID:17587762

Abstract

BACKGROUND

HDL-C has emerged as an important independent predictor of cardiovascular disease. The FNRI-HDL and NNHes Study Group in the Philippines reported that there was a high prevalence of low HDL among Filipinos. Most cases of low HDL-C are associated with secondary causes like Metabolic Syndrome. A primary cause of reduced HDL-C such as increase Cholesteryl Ester Transfer Protein activity has been identified.

OBJECTIVES

To determine the phenotype and frequency of Cholesteryl Ester Transfer Protein (TaqIB) polymorphism among Filipinos with cardiovascular risk factors. 2. To determine the association of TaqIB polymorphism with HDL-C levels among Filipinos with cardiovascular risk factors.

DESIGN

Cross-sectional Study.

SETTING

University of the Philippines-Philippine General Hospital.

STATISTICAL ANALYSIS

Descriptive statistics, Chi square test and Fisher's correlation test using Stata version 6.

METHODS

Fifty patients were included in this pilot study and were examined with respect to genotype, lipid profiles, blood sugar and other cardiovascular risk factors. Polymerase Chain Reaction (PCR), Restriction Fragment Length Polymorphism (RFLP) and Agarose Gel Electrophoresis techniques were used to determine the CETP TaqIB Polymorphism.

RESULTS

Out of 50 patients, 66% were females and 34% were males with a mean age of 55 y/o and a BMI of 27 kg/m(2). The following risk factors were identified: hypertension (92%), dyslipidemia (88%), obesity (68%), smoking (50%), diabetes mellitus type 2 (18%) and family history of premature CAD (14%). The genotype frequencies of B1B1; B1B2; B2B2 were 40%; 50% 10% respectively. The B1B1 homozygote was associated with lower HDL-C levels (45.35 +/- 8.82 mg/dL) compared to B1B2 (48.96 +/- 10.10 mg/dL) and B2B2 (48.99 +/- 10.13 mg/dL)).

CONCLUSIONS

Cholesteryl Ester Transfer Protein (TaqIB) Polymorphisms exist among Filipinos with cardiovascular risk factors. The frequency of TaqIB polymorphism among Filipinos with cardiovascular risk factors were B1B1 (40%), B1B2 (50%) and B2B2 (10%). B1B1 polymorphism is more common than B2B2 and associated with low HDL-C.

摘要

背景

高密度脂蛋白胆固醇（HDL-C）已成为心血管疾病的重要独立预测指标。菲律宾的食品和营养研究所高密度脂蛋白（FNRI-HDL）及非诺贝特与辛伐他汀联合治疗对高密度脂蛋白胆固醇及非高密度脂蛋白胆固醇影响的研究小组报告称，菲律宾人HDL水平低的患病率很高。大多数HDL-C水平低的病例与诸如代谢综合征等继发原因有关。已确定了HDL-C降低的一个主要原因，如胆固醇酯转运蛋白活性增加。

目的

确定有心血管危险因素的菲律宾人中胆固醇酯转运蛋白（TaqIB）多态性的表型和频率。2. 确定有心血管危险因素的菲律宾人中TaqIB多态性与HDL-C水平的关联。

设计

横断面研究。

地点

菲律宾大学菲律宾总医院。

统计分析

使用Stata 6版本进行描述性统计、卡方检验和费舍尔相关性检验。

方法

本初步研究纳入了50名患者，并对其基因型、血脂谱、血糖和其他心血管危险因素进行了检查。采用聚合酶链反应（PCR）、限制性片段长度多态性（RFLP）和琼脂糖凝胶电泳技术来确定CETP TaqIB多态性。

结果

50名患者中，66%为女性，34%为男性，平均年龄55岁，体重指数为27kg/m²。确定了以下危险因素：高血压（92%）、血脂异常（88%）、肥胖（68%）、吸烟（50%）、2型糖尿病（18%）和早发冠心病家族史（14%）。B1B1、B1B2、B2B2的基因型频率分别为40%、50%、10%。与B1B2（48.96±10.10mg/dL）和B2B2（48.99±10.13mg/dL）相比，B1B1纯合子与较低的HDL-C水平（45.35±8.82mg/dL）相关。