Cöplü Nilay, Gözalan Ayşegül, Akin Levent
Refik Saydam Hifzisihha Merkezi Başkanliği, Salgin Hastaliklar Araştirma Müdürlüğü, Ankara, Turkey.
Turkiye Parazitol Derg. 2007;31(2):123-8.
In coproparasitological examination, there is need to perform concentration methods in order to increase the probability of finding helminth eggs and protozoa cysts in addition to the widely used direct examination, especially when they are low in number. In this study, seven concentration methods were investigated on 134 fecal specimens as to their conformity of test results, difficulties in performing the test or interpretation, the time required and the cost. The methods compared were flotation methods using ZnSO4, modified ZnSO4, saturated NaCl and Sheather's sugar flotation and sedimentation methods using Ritchie formalin-ether, modified Telemann and simple sedimentation. Statistical analysis was performed with kappa and McNemar tests. The procedure of the methods were more troublesome when sedimentation techniques were performed, interpretation was easy for helminth eggs but difficult for protozoa cysts with all of the methods, the time required varied between 7-50 minutes, and none of them were expensive. Every laboratory can make their own choices depending upon their conditions, but according to the results of this study, it is apparent that simple sedimentation techniques together with the modified ZnSO4 flotation method would increase the success of the laboratory in diagnosis.
在粪便寄生虫学检查中,除了广泛使用的直接检查法外,还需要采用浓缩方法,以提高发现蠕虫卵和原生动物包囊的概率,尤其是当它们数量较少时。在本研究中,对134份粪便标本采用了七种浓缩方法,考察了它们在检测结果一致性、操作或判读难度、所需时间和成本方面的情况。所比较的方法包括使用硫酸锌、改良硫酸锌、饱和氯化钠和谢弗氏糖浮选法的浮选法,以及使用里奇氏甲醛乙醚法、改良泰勒曼法和简单沉淀法的沉淀法。采用kappa检验和麦克尼马尔检验进行统计分析。沉淀技术操作时方法步骤更繁琐,所有方法对蠕虫卵的判读容易,但对原生动物包囊的判读困难,所需时间在7至50分钟之间,且均成本不高。每个实验室可根据自身条件做出选择,但根据本研究结果,显然简单沉淀技术与改良硫酸锌浮选法相结合可提高实验室的诊断成功率。