体外非转铁蛋白结合铁对红系及粒-巨噬细胞集落形成的抑制作用：脱铁转铁蛋白的保护作用
Inhibition of erythroid and granulocyte-macrophage colony formation by non-transferrin-bound iron in vitro: protective effect of apotransferrin.
作者信息
Juvonen Eeva, Sahlstedt Leila, Parkkinen Jaakko, Ruutu Tapani
机构信息
Department of Medicine, Helsinki University Central Hospital, Helsinki, Finland.
出版信息
Eur J Haematol. 2007 Aug;79(2):126-31. doi: 10.1111/j.1600-0609.2007.00887.x. Epub 2007 Jun 27.
OBJECTIVES
The aim of the study was to investigate in vitro the effect of free iron on erythroid and granulocyte-macrophage colony formation and the effect of binding free iron with apotransferrin.
METHODS
Normal haematopoietic progenitors were cultured in vitro with different concentrations of free iron in the form of ferric nitrilotriacetic acid (FeNTA). Parallel cultures were performed after the preincubation of FeNTA with apotransferrin.
RESULTS
Free iron inhibited colony formation by erythroid and granulocyte-macrophage progenitors and reduced the size of the colonies in a dose-dependent manner. Preincubation of FeNTA with apotransferrin diminished the inhibitory effect of FeNTA on colony formation increasing both the number and the size of colonies.
CONCLUSIONS
Free iron was toxic to haematopoietic progenitors in in vitro cultures; the toxic effect could be reduced with apotransferrin.
目的
本研究旨在体外研究游离铁对红系和粒-巨噬细胞集落形成的影响以及脱铁转铁蛋白结合游离铁的作用。
方法
正常造血祖细胞在体外与不同浓度以次氮基三乙酸铁（FeNTA）形式存在的游离铁一起培养。在FeNTA与脱铁转铁蛋白预孵育后进行平行培养。
结果
游离铁抑制红系和粒-巨噬细胞祖细胞的集落形成，并以剂量依赖方式减小集落大小。FeNTA与脱铁转铁蛋白预孵育可减弱FeNTA对集落形成的抑制作用，增加集落数量和大小。
结论
在体外培养中游离铁对造血祖细胞有毒性；脱铁转铁蛋白可降低这种毒性作用。
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