激发素基因在种间杂种卵菌纲植物疫霉中的分布与表达
Distribution and expression of elicitin genes in the interspecific hybrid oomycete Phytophthora alni.
作者信息
Ioos Renaud, Panabières Franck, Industri Benoît, Andrieux Axelle, Frey Pascal
机构信息
INRA, Nancy-Université, UMR1136, Interactions Arbres-Microorganismes, F-54280 Champenoux, France.
出版信息
Appl Environ Microbiol. 2007 Sep;73(17):5587-97. doi: 10.1128/AEM.00721-07. Epub 2007 Jun 29.
Phytophthora alni subsp. alni, P. alni subsp. multiformis, and P. alni subsp. uniformis are responsible for alder disease in Europe. Class I and II elicitin gene patterns of P. alni subsp. alni, P. alni subsp. multiformis, P. alni subsp. uniformis, and the phylogenetically close species P. cambivora and P. fragariae were studied through mRNA sequencing and 3' untranslated region (3'UTR)-specific PCRs and sequencing. The occurrence of multiple 3'UTR sequences in association with identical elicitin-encoding sequences in P. alni subsp. alni indicated duplication/recombination events. The mRNA pattern displayed by P. alni subsp. alni demonstrated that elicitin genes from all the parental genomes are actually expressed in this allopolyploid taxon. The complementary elicitin patterns resolved confirmed the possible involvement of P. alni subsp. multiformis and P. alni subsp. uniformis in the genesis of the hybrid species P. alni subsp. alni. The occurrence of multiple and common elicitin gene sequences throughout P. cambivora, P. fragariae, and P. alni sensu lato, not observed in other Phytophthora species, suggests that duplication of these genes occurred before the radiation of these species.
欧洲桤木疫霉亚种桤木疫霉、多形桤木疫霉和整齐桤木疫霉可引发桤木病害。通过mRNA测序以及3'非翻译区（3'UTR）特异性PCR和测序，研究了桤木疫霉亚种桤木疫霉、多形桤木疫霉、整齐桤木疫霉以及系统发育关系相近的物种食杉疫霉和草莓疫霉的I类和II类激发素基因模式。桤木疫霉亚种桤木疫霉中存在多个与相同激发素编码序列相关的3'UTR序列，表明发生了重复/重组事件。桤木疫霉亚种桤木疫霉所呈现的mRNA模式表明，来自所有亲本基因组的激发素基因实际上在这个异源多倍体分类群中表达。解析得到的互补激发素模式证实了多形桤木疫霉亚种和整齐桤木疫霉亚种可能参与了杂交物种桤木疫霉亚种桤木疫霉的起源。在食杉疫霉、草莓疫霉和广义桤木疫霉中发现了多个常见的激发素基因序列，而在其他疫霉物种中未观察到，这表明这些基因的重复发生在这些物种分化之前。
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