Han Xuan-Mao, Ren Jin-Fang, Hao Bin, Cao Wen-Dong, Liu Xiu-E, Hou Li-Hong, Guo Zhi-Ping, Yu Bin, Wang Xue-Feng, Ding Qiu-Lan, Yang Lin-Hua
Department of Hematology, The Second Hospital, Shanxi Medical University, Taiyuan 030001, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Jun;15(3):612-6.
The study was aimed to investigate the factor V coagulation activity (FV:C), and to evaluate FVgene polymorphisms and activated protein C resistance (APCR) in the patients with venous thromboembolism (VTE). 95 patients with VTE and 95 normal controls were investigated for FV gene polymorphisms. FV Leiden, FVCambridge, and FVHong Kong were detected by PCR, MnlI and BstNI digestion respectively. FVAsp79His and FVI359T were detected by MassARRAY. FV:C and APCR in 65 patients with VTE and 60 normal controls were determined by a one-stage clotting method and the APTT-based assays respectively. The results showed that the mean levels of plasma FV:C were significantly higher in VTE group than that in controls (108.03% +/- 28.29% vs 95.17% +/- 29.75%) (P = 0.008), the incidence of APCR were 20.0% (13 of 65 cases) in patients with VTE and 5.0% (3 of 60 cases) in normal controls (P = 0.012). FV Leiden, FVCambridge, FVHong Kong, FVAsp79His and FVI359T mutations were not found in two groups. It is concluded that the increased plasma level of FV:C is a risk factor for VTE. There is APCR in both groups, APCR is also a risk factor to VTE. APCR may not be associated with mutations of FV Leiden, FVCambridge, FVHong Kong, FVAsp79His and FV I359T polymorphisms, other factors need to study further in APCR.
本研究旨在调查凝血因子V的凝血活性（FV:C），并评估静脉血栓栓塞症（VTE）患者的FV基因多态性和活化蛋白C抵抗（APCR）情况。对95例VTE患者和95例正常对照者进行FV基因多态性检测。分别采用聚合酶链反应（PCR）、MnlI酶切和BstNI酶切检测FV Leiden、FV Cambridge和FV Hong Kong。采用MassARRAY检测FVAsp79His和FVI359T。分别采用一期凝血法和基于活化部分凝血活酶时间（APTT）的检测方法测定65例VTE患者和60例正常对照者的FV:C和APCR。结果显示，VTE组血浆FV:C平均水平显著高于对照组（108.03%±28.29% vs 95.17%±29.75%）（P = 0.008），VTE患者APCR发生率为20.0%（65例中的13例），正常对照者为5.0%（60例中的3例）（P = 0.012）。两组均未发现FV Leiden、FV Cambridge、FV Hong Kong、FVAsp79His和FVI359T突变。结论：血浆FV:C水平升高是VTE的危险因素。两组均存在APCR，APCR也是VTE的危险因素。APCR可能与FV Leiden、FV Cambridge、FV Hong Kong、FVAsp79His和FV I359T多态性突变无关，APCR中的其他因素有待进一步研究。
