Pachter B R, Eberstein A
Department of Rehabilitation Medicine, New York University Medical Center, NY 10016.
Brain Res. 1991 Sep 27;560(1-2):311-4. doi: 10.1016/0006-8993(91)91249-z.
The incidence of motor nerve and terminal sprouting was quantitatively analyzed in normal unoperated muscles, in homologous muscles contralateral to muscles which have been partially denervated, in partially denervated muscles, and in sham-operated muscles. Muscles were studied by light microscopy after staining motor endplates by a combined silver-cholinesterase stain. In addition, the incidence of endplates containing terminal sprouts, the number of terminal branch points per endplate, and endplate, and endplate size were also assessed in the various groups examined. We observed that following section of the L4 spinal nerve, the incidence of sprouting (preterminal and intranodal) in the contralateral muscle exhibited a 2-fold increase over sham-operated controls. We also found a correlation between nerve terminal sprouting, terminal branch point number and endplate size. All of these parameters were significantly increased in the contralateral muscles as compared to the sham-operated control muscles. These findings suggest that normal muscles undergo sprouting which can be enhanced by contralateral partial denervation. The possible underlying mechanism may be the transneuronal induction of sprouting.
对正常未手术的肌肉、部分失神经支配肌肉对侧的同源肌肉、部分失神经支配的肌肉以及假手术肌肉中的运动神经和终末芽生发生率进行了定量分析。通过联合银胆碱酯酶染色对运动终板进行染色后，用光镜研究肌肉。此外，还评估了不同检查组中含有终末芽的终板发生率、每个终板的终末分支点数以及终板大小。我们观察到，切断L4脊神经后，对侧肌肉中芽生（终末前和结内）的发生率比假手术对照组增加了2倍。我们还发现神经终末芽生、终末分支点数与终板大小之间存在相关性。与假手术对照肌肉相比，对侧肌肉中的所有这些参数均显著增加。这些发现表明，正常肌肉会发生芽生，对侧部分失神经支配可增强这种芽生。潜在的机制可能是跨神经元的芽生诱导。
