Khanal Basudha, Sharma Sanjib Kumar, Bhattacharya Shyamal Kumar, Bhattarai Narayan Raj, Deb Monorama, Kanungo Reba
Department of Microbiology, B.P. Koirala Institute of Health Sciences, Dharan, Nepal.
J Health Popul Nutr. 2007 Mar;25(1):82-7.
The aim of the present study was to evaluate antimicrobial susceptibility patterns with special reference to multidrug resistance, susceptibility to ciprofloxacin, and bacteriophage typing of Salmonella enterica serotype Typhi isolated from blood sent for culture in a tertiary-care teaching hospital in eastern Nepal during January 2000-December 2004. In total, 132 strains of S. enterica Typhi, isolated from 2,568 blood culture samples collected from cases of suspected enteric fever, were tested for susceptibility to commonly-used antimicrobials by the disc-diffusion method. There were 35 multidrug-resistant strains. None of the isolates were resistant to ciprofloxacin. Of 52 isolates tested for minimum inhibitory concentration (MIC) of ciprofloxacin, 36 (69.23%) showed reduced susceptibility (MIC >0.25 mg/L). Of 112 strains tested for nalidixic acid susceptibility, 86 (76%) were resistant. Strains with reduced susceptibility to ciprofloxacin and resistance to nalidixic acid could be correlated. The commonest phage type was El. Nalidixic acid susceptibility could be a useful screening test for the detection of decreased susceptibility of S. Typhi to ciprofloxacin, a drug which is commonly used even for minor ailments in this area.
本研究的目的是评估2000年1月至2004年12月期间从尼泊尔东部一家三级护理教学医院送检血培养的伤寒沙门氏菌肠炎血清型伤寒杆菌的抗菌药敏模式，特别关注多重耐药性、对环丙沙星的敏感性以及噬菌体分型。总共从2568例疑似肠热症患者采集的血培养样本中分离出132株伤寒沙门氏菌肠炎血清型伤寒杆菌，采用纸片扩散法检测其对常用抗菌药物的敏感性。有35株多重耐药菌株。所有分离株均对环丙沙星不耐药。在52株检测环丙沙星最低抑菌浓度（MIC）的分离株中，36株（69.23%）显示敏感性降低（MIC>0.25mg/L）。在112株检测萘啶酸敏感性的菌株中，86株（76%）耐药。环丙沙星敏感性降低和萘啶酸耐药的菌株之间存在相关性。最常见的噬菌体类型是El。萘啶酸敏感性检测可能是一种有用的筛选试验，用于检测伤寒杆菌对环丙沙星敏感性降低，环丙沙星在该地区甚至常用于治疗小病。
