通过乳糖启动子对硝酸盐呼吸进行人工控制，有助于评估铜绿假单胞菌中氧介导的硝酸盐操纵子的翻译后调控。
Artificial control of nitrate respiration through the lac promoter permits the assessment of oxygen-mediated posttranslational regulation of the nar operon in Pseudomonas aeruginosa.
作者信息
Noriega Chris E, Sharma Vandana, Rowe John J
机构信息
Department of Biology, University of Dayton, 300 College Park, Dayton, OH 45469-2320, USA.
出版信息
J Bacteriol. 2007 Sep;189(17):6501-5. doi: 10.1128/JB.00491-07. Epub 2007 Jul 6.
Abstract
In this study, oxygen and nitrate regulation of transcription and subsequent protein expression of the unique narK1K2GHJI respiratory operon of Pseudomonas aeruginosa were investigated. Under the control of PLAC, P. aeruginosa was able to transcribe nar and subsequently express methyl viologen-linked nitrate reductase activity under aerobic conditions without nitrate. Modulation of PLAC through the LacI repressor enabled us to assess both transcriptional and posttranslational regulation by oxygen during physiological whole-cell nitrate reduction.
摘要
在本研究中，对铜绿假单胞菌独特的narK1K2GHJI呼吸操纵子转录及随后的蛋白质表达的氧和硝酸盐调控进行了研究。在PLAC的控制下，铜绿假单胞菌能够转录nar，并随后在有氧无硝酸盐条件下表达甲基紫精连接的硝酸盐还原酶活性。通过LacI阻遏物对PLAC进行调控，使我们能够评估在生理性全细胞硝酸盐还原过程中氧对转录和翻译后水平的调控。
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