McHaffie J G, Beninato M, Stein B E, Spencer R F
Department of Physiology, Medical College of Virginia, Richmond 23298.
J Comp Neurol. 1991 Nov 1;313(1):113-31. doi: 10.1002/cne.903130109.
The postnatal development of cholinergic afferents to the superior colliculus in neonatal cats was studied by using acetylcholinesterase (AChE) histochemistry, choline acetyltransferase (ChAT) immunohistochemistry, and retrograde transport of horseradish peroxidase (HRP). In the adult cat, the pattern of AChE staining was laminar specific. AChE was distributed continuously in the stratum griseum superficiale (SGS) but was organized as patches in the stratum griseum intermediate (SGI). Diffuse AChE staining also was present in the stratum griseum profundum (SGP) and the dorsolateral periaqueductal gray (PAG). At birth, however, AChE staining was barely detectable in the SGS and, aside from a few isolated labeled neurons, was absent from the SGI, SGP, and PAG. By 7 days postnatal (dpn), staining in the SGS was more apparent but did not change appreciably in the deeper laminae. A substantial increase in AChE staining occurred in the SGS at 14 dpn (several days after eye opening), at which time patches in the SGI first became apparent. By 28 dpn, the complete laminar-specific adult AChE staining pattern was present, though the staining intensity did not reach the adult level until 56 dpn. A protracted maturation of both AChE staining and ChAT immunoreactivity also was observed in the sources of cholinergic afferents to the superior colliculus, which include the parabigeminal nucleus, and the pedunculopontine (PPN) and lateral dorsal tegmental (LDTN) nuclei. AChE and ChAT-immunoreactive staining in each nucleus was weak at birth but increased during the ensuing 2 weeks. At 21 dpn, however, ChAT immunoreactivity virtually disappeared in the parabigeminal nucleus and significantly decreased in PPN and LDTN. The ChAT immunoreactivity in these nuclei then gradually increased reaching maximum levels by 28 dpn. At 35 dpn, AChE staining showed a significant, though temporary (4 weeks), decrease in the parabigeminal nucleus, but not in the PPN and LDTN, that subsequently increased to the adult level of staining at 70 dpn. The absence of AChE in the SGI in neonatal animals was correlated, at least in part, with a paucity of neurons in the brainstem cholinergic cell groups labeled by retrograde transport of HRP from the superior colliculus. Injections of HRP into the superior colliculus retrogradely labeled many neurons in the parabigeminal nucleus, but few, if any, neurons in the PPN or LDTN at 1 dpn. Retrogradely labeled neurons also were observed in the substantia nigra pars reticulata, albeit fewer in neonates than in adults.(ABSTRACT TRUNCATED AT 400 WORDS)
利用乙酰胆碱酯酶（AChE）组织化学、胆碱乙酰转移酶（ChAT）免疫组织化学以及辣根过氧化物酶（HRP）逆行运输技术，对新生猫上丘胆碱能传入纤维的出生后发育进行了研究。在成年猫中，AChE染色模式具有层特异性。AChE在浅层灰质（SGS）中连续分布，但在中间层灰质（SGI）中呈斑块状分布。在深层灰质（SGP）和背外侧导水管周围灰质（PAG）中也存在弥漫性AChE染色。然而，在出生时，SGS中几乎检测不到AChE染色，除了少数孤立的标记神经元外，SGI、SGP和PAG中均无AChE染色。出生后7天（dpn），SGS中的染色更明显，但深层板层中的染色没有明显变化。在14 dpn（睁眼后几天）时，SGS中的AChE染色显著增加，此时SGI中的斑块首次变得明显。到28 dpn时，已出现完整的层特异性成年AChE染色模式，不过染色强度直到56 dpn才达到成年水平。在上丘胆碱能传入纤维的来源，包括副视核、脚桥核（PPN）和外侧背被盖核（LDTN）中，也观察到AChE染色和ChAT免疫反应性的长期成熟过程。每个核中的AChE和ChAT免疫反应性染色在出生时较弱，但在随后的2周内增加。然而，在21 dpn时，ChAT免疫反应性在副视核中几乎消失，在PPN和LDTN中显著降低。这些核中的ChAT免疫反应性随后逐渐增加，到28 dpn时达到最高水平。在35 dpn时，AChE染色在副视核中显示出显著但短暂（4周）的减少，但在PPN和LDTN中没有，随后在70 dpn时增加到成年染色水平。新生动物SGI中缺乏AChE，至少部分与从脑干胆碱能细胞群逆行运输HRP标记的神经元数量稀少有关。将HRP注入上丘，在1 dpn时逆行标记了副视核中的许多神经元，但在PPN或LDTN中标记的神经元很少（如果有的话）。在黑质网状部也观察到逆行标记的神经元，尽管新生动物中的数量比成年动物少。（摘要截短至400字）
