[土生克雷伯菌E6与坚强芽孢杆菌E3细菌联合体的生理生化特性]

[Physiological and biochemical properties of the bacterial association of Klebsiella terrigena E6 and Bacillus firmus E3].

作者信息

Zlotnikov A K, Kazakov M L, Zlotnikov K M, Kazakov A V, Umarov M M

出版信息

Prikl Biokhim Mikrobiol. 2007 May-Jun;43(3):338-46.

PMID:17619582

Abstract

Physiological and biochemical properties of the natural rhizospheric association of Klebsiella terrigena E6 and Bacillusfirmus E3 were studied. It was demonstrated that this association fixed actively molecular nitrogen at a rate of 110 nmol ethylene per 1 mg protein per h. The dynamics of bacterial population in the association and in pure cultures were studied. The properties of this association depended on the ratio of its components; the maximal nitrogen fixation was recorded at a content of B. firmus E310 amounting to 15% of the total cell number. It was demonstrated that the stability basis of this association was the specific interactions via its components via metabolites--phenol and para-hydroxybenzoic acid--as well as via nitrogen fixation, respiration, and pH of the medium. A scheme of the interactions between the components of association is shown.

摘要

研究了土生克雷伯氏菌E6和坚强芽孢杆菌E3自然根际联合体的生理生化特性。结果表明，该联合体以每小时每毫克蛋白质110纳摩尔乙烯的速率积极固定分子态氮。研究了联合体和纯培养物中细菌群体的动态变化。该联合体的特性取决于其组分的比例；当坚强芽孢杆菌E310的含量占细胞总数的15%时，记录到最大固氮量。结果表明，该联合体稳定性的基础是其组分之间通过代谢产物——苯酚和对羟基苯甲酸——以及通过固氮、呼吸作用和培养基pH值进行的特异性相互作用。展示了联合体各组分之间相互作用的示意图。