Yao Chen, Li Xinsong, Song Tangying
Biomaterials and Drug Delivery Laboratories, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210018, P. R. China.
J Biomater Sci Polym Ed. 2007;18(6):731-42. doi: 10.1163/156856207781034070.
Electrospinning of biopolymers, such as proteins and polysaccharides, has recently attracted much attention for the fabrication of scaffolds for tissue engineering. In this report, zein/hyaluronic acid (HA) blend fibrous membranes were electrospun and characterized. To facilitate the compatibility of zein and HA, poly(vinyl pyrrolidone) (PVP) was introduced into aqueous ethanol solutions of the blend. A series of zein/HA/PVP blend fibrous membranes with different volume ratios were successfully electrospun. The effect of blend composition on the morphology of electrospun fibrous membranes was investigated by scanning electron microscopy. The average diameter of blend fibers increased with increasing the content of zein component. The electrospun zein/HA/PVP fibrous membranes were then cross-linked by methylene diphenyl diisocyanate (MDI). The morphology of the cross-linked zein/HA/PVP fibrous membranes changed slightly compared with un-cross-linked membranes. Tensile tests demonstrated that the mechanical properties of the zein/HA/PVP fibrous membranes were improved by cross-linking.
蛋白质和多糖等生物聚合物的静电纺丝最近在用于组织工程的支架制造方面备受关注。在本报告中，对玉米醇溶蛋白/透明质酸（HA）共混纤维膜进行了静电纺丝并表征。为促进玉米醇溶蛋白和HA的相容性，将聚乙烯吡咯烷酮（PVP）引入共混物的乙醇水溶液中。成功静电纺制出一系列具有不同体积比的玉米醇溶蛋白/HA/PVP共混纤维膜。通过扫描电子显微镜研究了共混物组成对静电纺纤维膜形态的影响。共混纤维的平均直径随着玉米醇溶蛋白组分含量的增加而增大。然后用二苯基甲烷二异氰酸酯（MDI）对静电纺的玉米醇溶蛋白/HA/PVP纤维膜进行交联。与未交联的膜相比，交联后的玉米醇溶蛋白/HA/PVP纤维膜的形态变化不大。拉伸试验表明，交联提高了玉米醇溶蛋白/HA/PVP纤维膜的力学性能。
