海胆卵匀浆的成分决定了其对肌醇三磷酸和环磷酸腺苷核糖诱导的钙离子释放的效力。

Composition of sea urchin egg homogenate determines its potency to inositol trisphosphate and cyclic ADPRibose-induced Ca2+ release.

作者信息

Jones Keith T, Swann Karl

机构信息

Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle NE2 4HH, UK.

出版信息

Biochem Biophys Res Commun. 2007 Sep 7;360(4):815-20. doi: 10.1016/j.bbrc.2007.06.134. Epub 2007 Jul 5.

DOI:10.1016/j.bbrc.2007.06.134

PMID:17624299

Abstract

Of the three intracellular Ca2+ signalling molecules (InsP3, cADPR and NAADP) sea urchin egg homogenate has been used in the identification and characterisation of two, cADPR and NAADP. Homogenate is prepared in a Na+/Cl- substitute of N-Methyl glucamine (NMG)/gluconate. To determine how media composition affects Ca2+ release we replaced NMG with various sugars or glycine and found a dramatic improvement in InsP3 mediated Ca2+ release. Conversely the response to cADPR was diminished, whilst NAADP was unaffected. Therefore modifying media composition may be an important consideration in using homogenate to study Ca2+ release for future studies.

摘要

在三种细胞内钙离子信号分子（三磷酸肌醇、环磷酸腺苷二磷酸核糖和烟酰胺腺嘌呤二核苷酸磷酸）中，海胆卵匀浆已被用于两种分子（环磷酸腺苷二磷酸核糖和烟酰胺腺嘌呤二核苷酸磷酸）的鉴定和特性研究。匀浆是在N-甲基葡糖胺（NMG）/葡萄糖酸盐的Na⁺/Cl⁻替代物中制备的。为了确定培养基成分如何影响钙离子释放，我们用各种糖类或甘氨酸替代了NMG，发现三磷酸肌醇介导的钙离子释放有显著改善。相反，对环磷酸腺苷二磷酸核糖的反应减弱，而烟酰胺腺嘌呤二核苷酸磷酸不受影响。因此，在未来的研究中，使用匀浆研究钙离子释放时，改变培养基成分可能是一个重要的考虑因素。