Navarro Ronald R, Ichikawa Hiroyasu, Iimura Yosuke, Tatsumi Kenji
National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan.
Environ Sci Technol. 2007 Jun 15;41(12):4240-5. doi: 10.1021/es0624523.
An aqueous DNA solution was applied for the extraction of polycyclic aromatic hydrocarbons (PAHs) from a spiked soil. Solubilities of 0.56, 11.78, and 11.24 mg L(-1) for anthracene, phenanthrene, and pyrene, respectively, were achieved after 1 day equilibration in 1% DNA. Using a spiked soil that contained 72 mg kg(-1) anthracene, 102 mg kg(-1) phenanthrene, and 99 mg kg(-1) pyrene, extractions of close to 88, 78, and 94%, respectively, were attained with 5% DNA at a 1:50 soil/extractant ratio. Maximum PAH dissolution occurred after 4-6 h. Comparative tests showed the main advantage of DNA over methyl-beta- and gamma-cyclodextrins and Tween 80 for pyrene removal. An ionic strength of 0.1 M NaCl was found necessary for maximum PAH dissolution and extraction. The performance of hexane regenerated DNA also remained stable after three stages of recycling. These results show the huge potential of DNA as an aqueous washing agent for PAH-contaminated soil.
将DNA水溶液用于从加标土壤中提取多环芳烃（PAHs）。在1%的DNA中平衡1天后，蒽、菲和芘的溶解度分别达到0.56、11.78和11.24 mg L⁻¹。使用含有72 mg kg⁻¹蒽、102 mg kg⁻¹菲和99 mg kg⁻¹芘的加标土壤，在土壤/萃取剂比例为1:50的情况下，用5%的DNA分别实现了接近88%、78%和94%的提取率。PAH的最大溶解在4 - 6小时后发生。对比试验表明，在去除芘方面，DNA相对于甲基-β-和γ-环糊精以及吐温80具有主要优势。发现0.1 M NaCl的离子强度对于PAH的最大溶解和提取是必要的。己烷再生的DNA在三个循环阶段后性能也保持稳定。这些结果表明DNA作为PAH污染土壤的水洗剂具有巨大潜力。
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