Guo Qiang, Li Shurong, Su Bingyin
Department of Neurobiology, Third Military Medical University, Chongqing 400038, PR China.
Neurosci Lett. 2007 Jul 11;422(2):103-8. doi: 10.1016/j.neulet.2007.05.034. Epub 2007 May 25.
Oligodendrocyte myelin glycoprotein (OMgp) is one kind of myelin-derived inhibitor, which strongly inhibits axonal regeneration through binding to its receptor NgR after the injury to the adult central nervous system (CNS). However, expression of OMgp and NgR after the adult spinal cord injury (SCI) remains unclear. Study on these problems will help to understand more comprehensively about the functions of these proteins in CNS during regeneration. Here, by using immunohistochemistry and reverse transcriptase PCR (RT-PCR), we found that after SCI, both OMgp and NgR expressions were detected in neurons and oligodendrocytes with a similar change profile that increased immediately and decreased gradually. We also found that the expression of OMgp is not limited in oligodendrocytes and its receptor NgR is not limited in neurons. They both can be expressed by these two kinds of cells. The roles of these factors in CNS regeneration require further study.
少突胶质细胞髓鞘糖蛋白（OMgp）是一种髓鞘源性抑制因子，在成体中枢神经系统（CNS）损伤后，它通过与受体NgR结合而强烈抑制轴突再生。然而，成体脊髓损伤（SCI）后OMgp和NgR的表达情况仍不清楚。对这些问题的研究将有助于更全面地了解这些蛋白质在CNS再生过程中的功能。在此，通过免疫组织化学和逆转录聚合酶链反应（RT-PCR），我们发现SCI后，在神经元和少突胶质细胞中均检测到OMgp和NgR的表达，且变化趋势相似，即损伤后立即升高，随后逐渐下降。我们还发现，OMgp的表达并不局限于少突胶质细胞，其受体NgR的表达也不局限于神经元。这两种细胞均可表达这两种蛋白。这些因子在CNS再生中的作用有待进一步研究。
