Shin Michael, Abukawa Harutsugi, Troulis Maria J, Vacanti Joseph P
Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
J Biomed Mater Res A. 2008 Mar 1;84(3):702-9. doi: 10.1002/jbm.a.31392.
Tissue engineering has been proposed as an approach to alleviate the shortage of donor tissue and organs by combining cells and a biodegradable scaffold as a temporary extracellular matrix. While numerous scaffold fabrication methods have been proposed, tissue formation is typically limited to the surface of the scaffolds in bone tissue engineering applications due to early calcification on the surface. To improve tissue formation, a novel scaffold with a hierarchical interconnected pore structure on two distinct length scales has been developed. Here we present the fabrication process and the application of the scaffold to bone tissue engineering. Porous poly(lactide-co-glycolide) (PLGA) scaffolds were made by combining solvent casting/particulate leaching with heat fusion. Porcine bone marrow-derived mesenchymal stem cells (MSCs) were differentiated into osteoblasts and cultured on these scaffolds in vitro for 2, 4, and 6 weeks. Subsequently, the constructs were assessed using histology and scanning electron microscopy. The bone marrow-derived osteoblasts attached well on these scaffolds. Cells were observed throughout the scaffolds. These initial results show promise for this scaffold to aid in the regeneration of bone.
组织工程学已被提出作为一种通过将细胞与可生物降解支架结合作为临时细胞外基质来缓解供体组织和器官短缺的方法。虽然已经提出了许多支架制造方法，但在骨组织工程应用中，由于支架表面早期钙化，组织形成通常局限于支架表面。为了改善组织形成，已开发出一种在两个不同长度尺度上具有分级互连孔结构的新型支架。在此，我们展示了该支架的制造过程及其在骨组织工程中的应用。多孔聚（丙交酯 - 乙交酯）（PLGA）支架通过溶剂浇铸/颗粒沥滤与热熔合相结合的方法制成。将猪骨髓间充质干细胞（MSCs）分化为成骨细胞，并在这些支架上体外培养2、4和6周。随后，使用组织学和扫描电子显微镜对构建体进行评估。骨髓来源的成骨细胞在这些支架上附着良好。在整个支架中都观察到了细胞。这些初步结果表明这种支架有助于骨再生。
