Torricelli Michela, Giovannelli Alessia, Leucci Eleonora, De Falco Giulia, Reis Fernando M, Imperatore Alberto, Florio Pasquale, Petraglia Felice
Department of Pediatrics, University of Siena, Siena, Italy.
Reprod Sci. 2007 Apr;14(3):241-5. doi: 10.1177/1933719107300971.
Because maternal plasma corticotrophin-releasing factor (CRF) levels increase during the last weeks of pregnancy and at parturition, the present study evaluated whether placental mRNA expression of CRF and CRF-binding protein (CRF-BP) are modified in preterm delivery. A group of 30 women with singleton pregnancies were enrolled in the study. A placental tissue specimen was collected from pregnant women (1) at term after cesarean delivery (39.3 +/- 0.1 gestational weeks; n = 10), (2) at term after spontaneous vaginal delivery (40.1 +/- 0.2 gestational weeks; n = 10), or (3) at preterm delivery (32.4 +/- 0.4 gestational weeks; n = 10). Changes of placental mRNA expression were evaluated by real-time quantitative reverse transcriptase polymerase chain reaction analysis. Placental CRF mRNA expression at term (P < .001) and preterm delivery (P < .001) was significantly higher than in cesarean delivery and highest in preterm placentas. With respect to CRF-BP, no significant difference in placental mRNA expression was observed among samples collected after term or preterm delivery and cesarean delivery. The present study showed for the first time that both term and preterm labor are associated with increased expression of placental CRF but not CRF-BP mRNA.
由于孕期最后几周及分娩时母体血浆促肾上腺皮质激素释放因子（CRF）水平会升高，本研究评估了早产时胎盘CRF及CRF结合蛋白（CRF-BP）的mRNA表达是否发生改变。30名单胎妊娠女性纳入本研究。从孕妇处采集胎盘组织样本：（1）剖宫产足月分娩后（孕周39.3±0.1周；n = 10），（2）自然阴道分娩足月后（孕周40.1±0.2周；n = 10），或（3）早产时（孕周32.4±0.4周；n = 10）。通过实时定量逆转录聚合酶链反应分析评估胎盘mRNA表达的变化。足月时（P <.001）及早产时（P <.001）胎盘CRF mRNA表达显著高于剖宫产分娩，且早产胎盘最高。关于CRF-BP，足月或早产及剖宫产分娩后采集的样本中，胎盘mRNA表达未观察到显著差异。本研究首次表明，足月和早产均与胎盘CRF而非CRF-BP mRNA表达增加有关。
