Inglese James, Johnson Ronald L, Simeonov Anton, Xia Menghang, Zheng Wei, Austin Christopher P, Auld Douglas S
US National Institutes of Health Chemical Genomics Center, National Institutes of Health, 9800 Medical Center Drive, Bethesda, Maryland 20892-3370, USA.
Nat Chem Biol. 2007 Aug;3(8):466-79. doi: 10.1038/nchembio.2007.17.
High-throughput screening (HTS) assays enable the testing of large numbers of chemical substances for activity in diverse areas of biology. The biological responses measured in HTS assays span isolated biochemical systems containing purified receptors or enzymes to signal transduction pathways and complex networks functioning in cellular environments. This Review addresses factors that need to be considered when implementing assays for HTS and is aimed particularly at investigators new to this field. We discuss assay design strategies, the major detection technologies and examples of HTS assays for common target classes, cellular pathways and simple cellular phenotypes. We conclude with special considerations for configuring sensitive, robust, informative and economically feasible HTS assays.
高通量筛选（HTS）分析能够对大量化学物质在生物学不同领域的活性进行检测。在HTS分析中测量的生物学反应范围广泛，从包含纯化受体或酶的孤立生化系统到信号转导途径以及在细胞环境中发挥作用的复杂网络。本综述探讨了实施HTS分析时需要考虑的因素，尤其针对该领域的新手研究人员。我们讨论了分析设计策略、主要检测技术以及针对常见靶标类别、细胞途径和简单细胞表型的HTS分析示例。我们最后阐述了配置灵敏、稳健、信息丰富且经济可行的HTS分析的特殊注意事项。
