Chen Xingguo, Fazal Md Abul, Dovichi Norman J
Department of Chemistry, University of Washington, Seattle, Washington, 98195, USA.
Talanta. 2007 Mar 30;71(5):1981-1985. doi: 10.1016/j.talanta.2006.09.004.
Two-dimensional capillary electrophoresis was used for the separation of proteins and biogenic amines from the mouse AtT-20 cell line. The first-dimension capillary contained a TRIS-CHES-SDS-dextran buffer to perform capillary sieving electrophoresis, which is based on molecular weight of proteins. The second-dimension capillary contained a TRIS-CHES-SDS buffer for micel1ar electrokinetic capillary chromatography. After a 61 seconds preliminary separation, fractions from the first-dimension capillary were successively transferred to the second-dimension capillary, where they further separated by MECC. The two-dimensional separation required 60 minutes.
二维毛细管电泳用于从小鼠AtT-20细胞系中分离蛋白质和生物胺。第一维毛细管含有TRIS-CHES-SDS-葡聚糖缓冲液,用于进行基于蛋白质分子量的毛细管筛分电泳。第二维毛细管含有用于胶束电动毛细管色谱的TRIS-CHES-SDS缓冲液。经过61秒的初步分离后,第一维毛细管中的馏分被依次转移到第二维毛细管中,在那里它们通过胶束电动毛细管色谱进一步分离。二维分离需要60分钟。