Zhao Zhi-Hong, Liao De-Xiang, Li Xiao-Ming, Zeng Guang-Ming, Yang Qi
College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
Huan Jing Ke Xue. 2007 Apr;28(4):800-4.
An SBR reactor seeded with anaerobic granular sludge was started-up to enrich ANAMMOX (anaerobic ammonia oxidation) bacteria and investigate its nitrogen removal characteristics. Research results showed that hydraulic retention time (HRT) was the most important factor to enrich ANAMMOX bacteria. When the HRT was controlled at 30 d during the initial cultivation, the SBR reactor presented ANAMMOX activity at t = 58 days. Simultaneously, the anaerobic granular sludge changed gradually from dust black to brownish color and its diameter became smaller. At t = 90 days, the ANAMMOX was obvious, and ANAMMOX granules was cultivated successfully. NH4(+) -N and NO2(-) -N were removed simultaneously with higher speed and maximum removal rate reached 14.6 g/(m3 x d) and 6.67 g/(m3 x d) respectively. Between t = 110 days and t = 156 days, the nitrogen load was increased gradually to a hydraulic retention time of 5 days. The removal rate of ammonium and nitrite were 60.6% and 62.5% respectively. The ratio of nitrite/ammonium was 1.12. The sludge changed to henna and formed ANAMMOX granulation with high nitrogen removal activity. The total nitrogen load was 34.3 g/(m3 x d).
启动了一个接种厌氧颗粒污泥的序批式反应器（SBR），以富集厌氧氨氧化（ANAMMOX）细菌并研究其脱氮特性。研究结果表明，水力停留时间（HRT）是富集ANAMMOX细菌的最重要因素。在初始培养期间，当HRT控制在30天时，SBR反应器在第58天呈现出ANAMMOX活性。同时，厌氧颗粒污泥逐渐从灰黑色变为褐色，其直径变小。在第90天，ANAMMOX现象明显，成功培养出ANAMMOX颗粒。NH4(+) -N和NO2(-) -N同时以较高速度被去除，最大去除率分别达到14.6 g/(m3·d)和6.67 g/(m3·d)。在第110天至第156天期间，氮负荷逐渐增加至水力停留时间为5天。铵和亚硝酸盐的去除率分别为60.6%和62.5%。亚硝酸盐/铵的比例为1.12。污泥变为棕红色并形成具有高脱氮活性的ANAMMOX颗粒。总氮负荷为34.3 g/(m3·d)。
