Shimada Toshio, Zilles Julie, Raskin Lutgarde, Morgenroth Eberhard
University of Illinois, Urbana-Champaign, 205 N. Mathews Ave., IL 61801, USA.
Water Res. 2007 Dec;41(20):4721-9. doi: 10.1016/j.watres.2007.06.052. Epub 2007 Jun 27.
This study demonstrates the accumulation and degradation of trehalose as a storage compound in a glucose-fed anaerobic sequencing batch reactor (ASBR). One hour after substrate addition, only 40% of the added organic matter (as chemical oxygen demand, COD) was accounted for by the cumulative methane production and soluble COD remaining in the reactor. All influent COD was accounted for by methane and biomass production by the end of the 24-h ASBR cycle. These dynamics can be explained by the production of an intracellular storage product. Total carbohydrate analysis showed that 26% of the glucose added to the reactor transiently accumulated within the biomass. Based on (13)C-nuclear magnetic resonance (NMR) analysis, trehalose (alpha-D-glucopyranosyl-(D-glucopyranoside)) was identified as the main carbohydrate produced. Mathematical modeling was performed and the IWA Anaerobic Digestion Model No. 1 (ADM1) was modified to include microbial storage. The modified model adequately described the ASBR dynamics during a 24-h cycle.
本研究展示了海藻糖作为一种储存化合物在以葡萄糖为底物的厌氧序批式反应器（ASBR）中的积累和降解情况。添加底物1小时后，通过累积甲烷产量和反应器中剩余的可溶性化学需氧量（COD）仅能解释所添加有机物（以COD计）的40%。在24小时的ASBR周期结束时，所有进水COD都通过甲烷和生物质产量得到了解释。这些动态变化可以通过细胞内储存产物的产生来解释。总碳水化合物分析表明，添加到反应器中的葡萄糖有26%暂时积累在生物质中。基于碳-13核磁共振（NMR）分析，海藻糖（α-D-吡喃葡萄糖基-(D-吡喃葡萄糖苷)）被确定为产生的主要碳水化合物。进行了数学建模，并对国际水协厌氧消化1号模型（ADM1）进行了修改，以纳入微生物储存。修改后的模型充分描述了24小时周期内ASBR的动态变化。
