[从住院患者和携带者分离出的金黄色葡萄球菌菌株中肠毒素基因的出现情况]

[Enterotoxin genes occurance among S. aureus strains isolated from inpatients and carriers].

作者信息

Lawrynowicz-Paciorek Maja, Kochman Maria, Piekarska Katarzyna, Wyrebiak Agata, Potracka Ewa, Leniak-Chmiel Urszula, Magdziak Agnieszka

机构信息

Zakład Bakteriologii Państwowego Zakładu Higieny w Warszawie.

出版信息

Med Dosw Mikrobiol. 2006;58(4):275-81.

PMID:17642305

Abstract

We examined 44 inpatients and 66 carriers Staphylococcus aureus strains, isolated in years 2002-2005, for the presence of 18 enterotoxin genes (se/sel) (by PCR), the ability for A-D enterotoxin production (by SET-RPLA) and antibiotic resistance distribution (by disc diffusion method). se/sel genes were detected in 90,9% of all strains, sea (70,5%) and selk and selq (52,3%) - among inpatients strains and egc (65,2%) - among carriers strains were the most frequently se/sel genes found. Positive results of SET-RPLA were consistent with PCR results. There was no correlation observed between antibiotic resistance and se/sel genes distribution among tested S. aureus strains.

摘要

我们检测了2002年至2005年间分离出的44株住院患者金黄色葡萄球菌菌株和66株携带者金黄色葡萄球菌菌株，以检测18种肠毒素基因（se/sel）的存在情况（采用聚合酶链反应法）、A-D型肠毒素的产生能力（采用SET-RPLA法）以及抗生素耐药性分布情况（采用纸片扩散法）。在所有菌株中，90.9%检测到se/sel基因，住院患者菌株中sea基因（70.5%）以及selk和selq基因（52.3%）最为常见，携带者菌株中egc基因（65.2%）最为常见。SET-RPLA的阳性结果与聚合酶链反应结果一致。在所检测的金黄色葡萄球菌菌株中，未观察到抗生素耐药性与se/sel基因分布之间存在相关性。