“开启”型柔性硫属代吡喃鎓光敏剂。与DNA结合后光物理性质的变化。
"Switched-on" flexible chalcogenopyrylium photosensitizers. Changes in photophysical properties upon binding to DNA.
作者信息
Ohulchanskyy Tymish Y, Gannon Michael K, Ye Mao, Skripchenko Andrey, Wagner Stephen J, Prasad Paras N, Detty Michael R
机构信息
Institute for Lasers, Photonics, and Biophotonics, Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, New York 14260, USA.
出版信息
J Phys Chem B. 2007 Aug 16;111(32):9686-92. doi: 10.1021/jp073270d. Epub 2007 Jul 24.
2,4-Bis(4-dimethylaminophenyl)-6-alkylthiopyrylium and selenopyrylium dyes are essentially nonfluorescent (phi F < 0.001) and are poor generators of singlet oxygen in aqueous solution. However, upon complexation to calf thymus DNA, quantum yields for both fluorescence and generation of singlet oxygen increased dramatically. Irradiation of the dye-DNA complexes produced strand breaks in the DNA. The photodamage is not observed in the absence of oxygen and is suppressed by the addition of the singlet oxygen quencher imidazole. The inactivation of the pseudo-rabies virus upon treatment of oxygenated leukodepleted 20% hematocrit red blood cell suspensions with the chalcogenopyrylium dyes and light followed the same trend observed with quantum yields for the generation of singlet oxygen in the dye-DNA complexes.
2,4-双（4-二甲基氨基苯基）-6-烷基硫代吡喃鎓和硒代吡喃鎓染料基本上无荧光（荧光量子产率φF < 0.001），且在水溶液中产生单线态氧的能力较差。然而，与小牛胸腺DNA络合后，荧光和单线态氧生成的量子产率均显著增加。染料-DNA复合物的辐照导致DNA链断裂。在无氧条件下未观察到光损伤，且添加单线态氧猝灭剂咪唑可抑制光损伤。用硫属元素吡喃鎓染料和光处理充氧的、去除白细胞的20%血细胞比容红细胞悬液时，伪狂犬病病毒的失活遵循与染料-DNA复合物中单线态氧生成量子产率相同的趋势。
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