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适用于现代技术的转基因植物实验模型

[Experimental models of transgenic plants promising for the modern technologies].

作者信息

Abdeeva I A, Goldenkova-Pavlova I V, Mokriakova M V, Volkova L V, Bogush V G, Sidoruk K V, Iur'eva N O, Debabov V G, Piruzian E S

出版信息

Tsitol Genet. 2007 May-Jun;41(3):55-61.

Abstract

Transgenic popato plants have been created which express recombinant proteins, analogues of spidroin 1, the protein of the cobweb skeleton thread. Expression of the hybrid spidroin 1 genes possessing some repeated sequences retains both in the model test-tube-growing plants and in the crops. Expression level of the synthetic spidroin 1 genes and the level of accumulation of their products in plants depend on the type of promoter, number of repeats, organ specificity and plant species but not on the duration of plant material storage. The results show that the strategy based on constuction and expression of hybrid proteins which include the reporter protein makes it easier to select and analyse expression of hybrid proteins in transgenic organisms.

摘要

已经培育出了转基因马铃薯植株,这些植株能够表达重组蛋白,即蛛丝蛋白1的类似物,蛛丝蛋白1是蜘蛛网骨架丝的蛋白质。具有一些重复序列的杂交蛛丝蛋白1基因在模式试管培养植物和作物中均能保留表达。合成蛛丝蛋白1基因的表达水平及其在植物中的产物积累水平取决于启动子类型、重复序列数量、器官特异性和植物种类,而不取决于植物材料的储存时间。结果表明,基于构建和表达包含报告蛋白的杂交蛋白的策略,使得在转基因生物中选择和分析杂交蛋白的表达变得更加容易。

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