谷物中瓜扎汀残留量定量检测的液相色谱/电喷雾电离/质谱法

LC/ESI/MS method for the quantitative detection of guazatine residues in cereals.

作者信息

Dreassi Elena, Zizzari Alessandra Tania, Zanfini Assunta, Corbini Gianfranco, Botta Maurizio

机构信息

Università degli Studi di Siena, Dipartimento Farmaco Chimico Tecnologico - via A. Moro, 53100 Siena, Italy.

出版信息

J Agric Food Chem. 2007 Aug 22;55(17):6850-6. doi: 10.1021/jf070648w. Epub 2007 Jul 25.

DOI:10.1021/jf070648w

PMID:17650002

Abstract

Guazatine is a fungicide used in agriculture to control a wide range of seed-borne diseases of cereals and other vegetable foods. In this work, a LC-ESI-MS method was developed for the quantitative detection of guazatine residues in maize and hard wheat. Quantitative data were determined for the residues of the main diamines, triamines, and tetramines that cover more than 87% of the total contents of the mixture. The mean recoveries from the fortified cereals at 0.050 mg/kg ranged from 81 to 86%, with the coefficients of variation (CVs) ranging from 0.9 to 5.5% (n = 5). At 0.025 mg/kg, the recoveries ranged from 78 to 87%, with the CVs ranging from 0.8 to 6.3% (n = 5). The limits of quantification have been estimated to be 0.010, 0.004, 0.002, 0.002, 0.005, and 0.002 mg/kg, respectively, for GN, GG, GNG, GGN, GGG, and GGGG in maize and hard wheat (S/N ratio >10).

摘要

多果定是一种用于农业的杀菌剂，用于防治谷物和其他蔬菜类食品的多种种传病害。在本研究中，开发了一种液相色谱-电喷雾电离质谱法（LC-ESI-MS），用于定量检测玉米和硬粒小麦中的多果定残留。测定了主要二胺、三胺和四胺残留的定量数据，这些成分占混合物总含量的87%以上。在添加浓度为0.050 mg/kg的谷物中，平均回收率为81%至86%，变异系数（CV）为0.9%至5.5%（n = 5）。在添加浓度为0.025 mg/kg时，回收率为78%至87%，CV为0.8%至6.3%（n = 5）。对于玉米和硬粒小麦中的GN、GG、GNG、GGN、GGG和GGGG，估计定量限分别为0.010、0.004、0.002、0.002、0.005和0.002 mg/kg（信噪比>10）。