[通过原生质体融合系统获得的蔊菜（Rorippa indica (Linn.) Hiern）与甘蓝型油菜（Brassica napus L.）的体细胞杂种]

[Somatic hybrids between Rorippa indica (Linn.) Hiern and Brassica napus L. through protoplast-fusion system].

作者信息

Jiang Shu-Hui, Guan Rong-Zhan, Tang San-Yuan, Xin Ru-Ying, Zhang Hong-Sheng, Zhao Li-Qian, Pan Qin-Yan

机构信息

State Key Lab of Crop Genetics and Germpalsm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Yi Chuan. 2007 Jun;29(6):745-50. doi: 10.1360/yc-007-0745.

DOI:10.1360/yc-007-0745

PMID:17650493

Abstract

High yield of protoplast isolation was achieved from hypocotyls of B. napus L. and leaves of Rorippa indica (Linn.) Hiern. The isolated protoplasts were used to establish an efficient protoplast-fusion system between the two cruciferous species by PEG-DMSO method and culturing with MS liquid medium. Ten somatic fusion hybrids between B. napus and R. indica were obtained. The enzyme combinations for isolating protoplast from B. napus L. and R. indica were 1% cellulase + 0.2% macerozyme + 3 mmol/L MES and 0.25% cellulase + 0.5% macerozyme + 5 mmol/L MES, respectively. Fusion percentage of 10.4% was obtained on the condition of 30% PEG + 0.3 mol/L glucose +50 mmol/L CaCl2.2H2O + 15% DMSO. Seeds plants obtained from protoplast fusion are new germplasm derived from R. indica.

摘要

从甘蓝型油菜的下胚轴和蔊菜的叶片中获得了高产的原生质体。分离得到的原生质体通过聚乙二醇-二甲基亚砜（PEG-DMSO）法并在MS液体培养基中培养，用于建立这两种十字花科物种之间高效的原生质体融合体系。获得了10个甘蓝型油菜和蔊菜之间的体细胞融合杂种。从甘蓝型油菜和蔊菜中分离原生质体的酶组合分别为1%纤维素酶+0.2%果胶酶+3 mmol/L MES和0.25%纤维素酶+0.5%果胶酶+5 mmol/L MES。在30% PEG + 0.3 mol/L葡萄糖+50 mmol/L氯化钙二水合物+15% DMSO的条件下，融合率达到了10.4%。从原生质体融合获得的种子植株是源自蔊菜的新种质。