Ji Guang, Wang Lei, Liu Tao
Laboratory of Hepatic Adipose Infiltration, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 May;27(5):421-5.
To observe the apoptosis of hepatic stellate cell (HSC) and hepatocyte and the effect of Qinggan Huoxue Recipe (QHR) on it in alcoholic liver fibrosis (ALF) rats.
Rats were divided into six groups: the normal group, the model group, the QHR low dose (4.75 g/(kg x d)], medium dose (14.25 g/(kg x d)] and high dose (28.5 g/(kg x d)) groups, and the Essentiale (66.5 mg/(kg x d)] group. They were treated with respective drugs through gavage for 2 weeks, while the normal and model groups were given normal saline instead. The serum levels of ALT, AST and gamma-GT were measured by chromatometry, the degree of inflammation and fibrosis was observed by HE staining and Masson staining, and the apoptosis of hepatocyte and HSC were detected by TUNEL and TUNEL-alpha-SMA double immunolabeling respectively.
Compared with those before treatment, the serum levels of ALT, AST and gamma-GT obviously decreased (P < 0.05), inflammation and fibrosis relieved (P < 0.05), and the apoptosis of hepatocyte reduced (P < 0.05), while that of activated HSC increased in the QHR groups after treatment (P <0.05).
QHR can effectively relieve hepatic fibrosis, decrease hepatocyte apoptosis caused by alcohol and induce activated HSC apoptosis in ALF rats.
观察酒精性肝纤维化（ALF）大鼠肝星状细胞（HSC）和肝细胞的凋亡情况以及清肝活血方（QHR）对其的影响。
将大鼠分为六组：正常组、模型组、QHR低剂量组（4.75 g/(kg·d)）、中剂量组（14.25 g/(kg·d)）、高剂量组（28.5 g/(kg·d)）和易善复组（66.5 mg/(kg·d)）。各组分别通过灌胃给予相应药物2周，正常组和模型组给予生理盐水。采用比色法测定血清谷丙转氨酶（ALT）、谷草转氨酶（AST）和γ-谷氨酰转肽酶（γ-GT）水平，通过苏木精-伊红（HE）染色和Masson染色观察炎症和纤维化程度，分别采用末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）和TUNEL-α平滑肌肌动蛋白（α-SMA）双重免疫标记法检测肝细胞和HSC的凋亡情况。
与治疗前相比，治疗后QHR各组血清ALT、AST和γ-GT水平明显降低（P < 0.05），炎症和纤维化减轻（P < 0.05），肝细胞凋亡减少（P < 0.05），而活化HSC凋亡增加（P < 0.05）。
清肝活血方可有效减轻ALF大鼠肝纤维化，减少酒精所致肝细胞凋亡，并诱导活化HSC凋亡。
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