利用fadA基因敲除突变体恶臭假单胞菌过量生产中链长度聚羟基脂肪酸酯。
Utilization of fadA knockout mutant Pseudomonas putida for overproduction of medium chain-length-polyhydroxyalkanoate.
作者信息
Vo Minh Tri, Lee Kwang-Woo, Kim Tae-Kwon, Lee Yong-Hyun
机构信息
Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, South Korea.
出版信息
Biotechnol Lett. 2007 Dec;29(12):1915-20. doi: 10.1007/s10529-007-9476-5. Epub 2007 Jul 25.
The fadBA operon in the fatty acid beta-oxidation pathway of P. putida KCTC1639 was blocked to induce a metabolic flux of the intermediates to the biosynthesis of medium chain-length PHA (mcl-PHA). Succinate at 150 mg l(-1) stimulated cell growth and also the biosynthesis of medium chain-length-polyhydroxyalkanoate. pH-stat fed-batch cultivation of the fadA knockout mutant P. putida KCTC1639 was carried out for 60 h, in which mcl-PHA reached 8 g l(-1) with a cell dry weight of 10.3 g l(-1).
恶臭假单胞菌KCTC1639脂肪酸β-氧化途径中的fadBA操纵子被阻断，以诱导中间体的代谢流进入中链长度聚羟基脂肪酸酯（mcl-PHA）的生物合成。150 mg l(-1)的琥珀酸刺激了细胞生长以及中链长度聚羟基脂肪酸酯的生物合成。对fadA基因敲除突变体恶臭假单胞菌KCTC1639进行pH-stat补料分批培养60小时，其中mcl-PHA达到8 g l(-1)，细胞干重为10.3 g l(-1)。
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