Liu Xuefen, Deng Zhongxiang, Gao Shi, Sun Xiaofen, Tang Kexuan
State Key Laboratory of Genetic Engineering, School of Life Sciences, Institute of Genetics, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, People's Republic of China.
DNA Seq. 2007 Oct;18(5):371-9. doi: 10.1080/10425170701389063.
Glutathione S-transferases (GSTs) play an important role in the response of plants to changing environmental conditions. Here, we report the cloning of the GST gene for GST from Ginkgo biloba, a native medicinal plant species in China, by rapid amplification of cDNA ends (RACE). The full-length cDNA (designated as GbGST) was 1008 bp and contained a 684 bp open reading frame (ORF) encoding a polypeptide of 228 amino acids. The genomic sequence of GbGST was also obtained. Semi-quantitative RT-PCR analysis revealed that GbGST expressed in all tested tissues of G. biloba, including leaf, root and stem and the expression of GbGST could be induced by UV, MJ and drought treatments, suggesting that GbGST was potentially involved in plant's stress tolerance. To our knowledge, this is the first GST cDNA cloned from Ginkgoaceae. Based on comparative analyses of amino acid sequence, phylogeny, predicted three-dimensional structure together with the gene structure, the GbGST should be classified into the tau class.
谷胱甘肽S-转移酶（GSTs）在植物应对不断变化的环境条件中发挥着重要作用。在此，我们报告了通过cDNA末端快速扩增（RACE）技术，从中国本土药用植物银杏中克隆GST基因的GST。全长cDNA（命名为GbGST）为1008 bp，包含一个684 bp的开放阅读框（ORF），编码一个228个氨基酸的多肽。还获得了GbGST的基因组序列。半定量RT-PCR分析表明，GbGST在银杏的所有测试组织中均有表达，包括叶、根和茎，并且GbGST的表达可被紫外线、茉莉酸甲酯和干旱处理诱导，这表明GbGST可能参与了植物的胁迫耐受性。据我们所知，这是从银杏科克隆的第一个GST cDNA。基于氨基酸序列、系统发育、预测的三维结构以及基因结构的比较分析，GbGST应归类为tau类。
