Behari J R, Gupta S, Srivastava S, Srivastava R C
Industrial Toxicology Research Centre, M.G. Marg, Lucknow, India.
J Microencapsul. 1991 Apr-Jun;8(2):215-20. doi: 10.3109/02652049109071489.
alpha-Tocopherol is a well-known membrane associated chain-breaking phenolic antioxidant which functions as a trap for peroxyl and other free radicals and thus inhibits lipid peroxidation of membranes. Antioxidative effect of alpha-tocopherol when incorporated in liposomes was examined by the generation of Thiobarbituric acid (TBA) reacting species in the presence of nickel. The incorporation of alpha-tocopherol in the lipid bilayer resulted in the enhancement of lipid peroxidation at low concentration (1.0 mg) but at higher concentration (2.5 and 5.0 mg) considerably reduced the enhancement in lipid peroxidation of liposomes in the presence of nickel. When alpha-tocopherol was added (unincorporated) to liposomes in the presence of nickel, enhancement in lipid peroxidation was observed compared to nickel alone. Thus the antioxidative effect of alpha-tocopherol in the liposomes may depend on its mode of incorporation in the lipid bilayer.
α-生育酚是一种著名的与膜相关的链断裂酚类抗氧化剂，它作为过氧自由基和其他自由基的捕获剂，从而抑制膜的脂质过氧化。通过在镍存在下硫代巴比妥酸（TBA）反应性物质的生成，研究了α-生育酚掺入脂质体时的抗氧化作用。在脂质双层中掺入α-生育酚，在低浓度（1.0毫克）时导致脂质过氧化增强，但在较高浓度（2.5和5.0毫克）时，在镍存在下显著降低了脂质体脂质过氧化的增强。当在镍存在下将α-生育酚（未掺入）添加到脂质体中时，与单独的镍相比，观察到脂质过氧化增强。因此，α-生育酚在脂质体中的抗氧化作用可能取决于其掺入脂质双层的方式。
