角叉菜胶对犬牙龈组织中花生四烯酸12-脂氧合酶活性的刺激作用。
Stimulation by carrageenan of arachidonate 12-lipoxygenase activity in dog gingival tissue.
作者信息
Tamai K, Dohi T, Yoshino H, Shirakawa M, Okamoto H, Tsujimoto A
机构信息
Department of Endodontology, Hiroshima University School of Dentistry, Japan.
出版信息
Arch Oral Biol. 1991;36(12):913-7. doi: 10.1016/0003-9969(91)90123-c.
At 4 h after injection of carrageenan into the gingiva, the 12-lipoxygenase activity of the gingival homogenate was markedly increased. Activity in the cytosol and microsomal fractions was markedly increased when assessed as the specific activity based on nmol/min/mg of protein, and in the cytosol fraction as the percentage distribution of total activity. The 12-lipoxygenase activity in the homogenate from carrageenan-treated gingiva was not affected by either EDTA or calcium ion, or a combination of the two. 12-lipoxygenase activity in both carrageenan-treated and untreated gingiva was inhibited dose-dependently by AA861, a striking difference from its effect on platelet 12-lipoxygenase. There was a marked increase of 12-lipoxygenase activity in experimentally inflamed gingiva compared to the non-inflamed gingiva.
将角叉菜胶注射到牙龈4小时后，牙龈匀浆的12-脂氧合酶活性显著增加。以基于nmol/分钟/毫克蛋白质的比活性评估时，细胞溶质和微粒体部分的活性显著增加，以细胞溶质部分总活性的百分比分布评估时也是如此。角叉菜胶处理的牙龈匀浆中的12-脂氧合酶活性不受EDTA或钙离子或两者组合的影响。AA861对角叉菜胶处理和未处理牙龈中的12-脂氧合酶活性均有剂量依赖性抑制作用，这与其对血小板12-脂氧合酶的作用有显著差异。与未发炎的牙龈相比，实验性发炎牙龈中的12-脂氧合酶活性显著增加。
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