Furlan Cláudia M, Moraes Regina M, Bulbovas Patricia, Sanz Maria J, Domingos Marisa, Salatino Antonio
Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, CP 11461, 05422-970 São Paulo, SP, Brazil.
Environ Pollut. 2008 Mar;152(2):361-5. doi: 10.1016/j.envpol.2007.06.042. Epub 2007 Aug 2.
Tibouchina pulchra saplings were exposed to carbon filtered air (CF), ambient non-filtered air (NF) and ambient non-filtered air+40 ppb ozone (NF+O3) 8 h per day during two months. The AOT40 values at the end of the experiment were 48, 910 and 12,895 ppb h(-1), respectively, for the three treatments. After 25 days of exposure (AOT40=3871 ppb h(-1)), interveinal red stippling appeared in plants in the NF+O3 chamber. In the NF chamber, symptoms were observed only after 60 days of exposure (AOT40=910 ppb h(-1)). After 60 days, injured leaves per plant corresponded to 19% in NF+O3 and 1% in the NF treatment; and the average leaf area injured was 7% within the NF+O3 and 0.2% within the NF treatment. The extent of leaf area injured (leaf injury index) was mostly explained by the accumulated exposure of ozone (r2=0.89; p<0.05).
在两个月的时间里，美丽紫背竹芋幼树每天有8小时暴露于经过碳过滤的空气（CF）、未经过滤的环境空气（NF）以及未经过滤的环境空气+40 ppb臭氧（NF+O3）中。实验结束时，三种处理的AOT40值分别为48、910和12,895 ppb h(-1)。暴露25天后（AOT40 = 3871 ppb h(-1)），NF+O3舱内的植株出现了脉间红色斑点。在NF舱中，仅在暴露60天后（AOT40 = 910 ppb h(-1)）才观察到症状。60天后，NF+O3处理中每株植物的受伤叶片为19%，NF处理中为1%；NF+O3处理中叶片平均受伤面积为7% , NF处理中为0.2%。叶面积受伤程度（叶损伤指数）主要由臭氧累积暴露量解释（r2 = 0.89；p<0.05）。
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