用纯化的F18菌毛对仔猪进行黏膜免疫不能预防F18 +大肠杆菌感染。
Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection.
作者信息
Verdonck F, Tiels P, van Gog K, Goddeeris B M, Lycke N, Clements J, Cox E
机构信息
Laboratory of Immunology, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820 Merelbeke, Belgium.
出版信息
Vet Immunol Immunopathol. 2007 Dec 15;120(3-4):69-79. doi: 10.1016/j.vetimm.2007.06.018. Epub 2007 Jun 22.
Post-weaning diarrhoea and oedema disease in weaned piglets are caused by infection with F4+ or F18+ Escherichia coli strains. There is no commercial vaccine available, but it is shown that oral immunization of weaned piglets with purified F4 fimbriae induces a protective mucosal immune response. In the present study, piglets were orally and nasally immunized with purified F18 fimbriae in the presence of the mucosal adjuvant LT(R192G) or CTA1-DD, respectively. This immunization could not lead to protection against F18+ E. coli infection. The induced F18-specific immune response was directed towards the major subunit FedA and weakly towards the adhesive subunit FedF. The results of these experiments demonstrate that it is difficult to induce protective immunity against F18+ E. coli using the whole fimbriae due to the low response against the adhesin.
断奶仔猪的断奶后腹泻和水肿病是由感染F4 +或F18 +大肠杆菌菌株引起的。目前尚无商业疫苗，但研究表明，用纯化的F4菌毛对断奶仔猪进行口服免疫可诱导保护性黏膜免疫反应。在本研究中，仔猪分别在黏膜佐剂LT(R192G)或CTA1-DD存在的情况下，通过口服和鼻腔途径用纯化的F18菌毛进行免疫。这种免疫不能产生针对F18 +大肠杆菌感染的保护作用。诱导产生的F18特异性免疫反应主要针对主要亚基FedA，而对黏附亚基FedF的反应较弱。这些实验结果表明，由于对黏附素的反应较低，使用完整菌毛难以诱导针对F18 +大肠杆菌的保护性免疫。
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