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使用扩散张量成像对多发性硬化症中外观正常的脑组织进行体内细胞定向分析。

Cell-oriented analysis in vivo using diffusion tensor imaging for normal-appearing brain tissue in multiple sclerosis.

作者信息

Terajima Kenshi, Matsuzawa Hitoshi, Tanaka Keiko, Nishizawa Masatoyo, Nakada Tsutomu

机构信息

Center for Integrated Human Brain Science, Brain Research Institute, University of Niigata, Niigata, Japan.

出版信息

Neuroimage. 2007 Oct 1;37(4):1278-85. doi: 10.1016/j.neuroimage.2007.06.026. Epub 2007 Aug 6.

Abstract

There have been several methods proposed so far using diffusion tensor imaging (DTI) for the assessment of normal-appearing brain tissue (NABT) injury in multiple sclerosis (MS). However, for these methods, the analyses of the NABT injury at the cellular level, wherein histological examinations can be used, still present challenging problems. We developed a method of segregating NABT into the following anatomical structures using lambda chart analysis associated with a two-dimensional Gaussian deconvolution of diffusion characteristic functions: 1) structures primarily composed of small neurons and glia; 2) structures primarily composed of large neurons; 3) structures primarily composed of short axons; and 4) structures primarily composed of long axons. Each segregated structure that had a distinctive diffusion characteristic was subjected to the statistical inference of DTI-derived parameters for 14 patients with conventional relapsing-remitting MS (RRMS) and 20 age-matched healthy volunteers. In all of the structures, the trace values were significantly higher and the fractional anisotropy values were significantly lower in the RRMS patients than in the healthy volunteers. Furthermore, the volume fractions of the structures primarily composed of short axons markedly decreased, whereas those of the structures primarily composed of small neurons and glia markedly increased. These results suggest that axonal loss and glial proliferation predominantly occurred in the subcortical white matter and adjacent deep cortical layer, namely, the juxtacortical region. This cell-oriented analysis of NABT injury using DTI confirmed in vivo the histological observation that the juxtacortical region is the most vulnerable site in MS.

摘要

到目前为止,已经提出了几种使用扩散张量成像(DTI)来评估多发性硬化症(MS)中外观正常的脑组织(NABT)损伤的方法。然而,对于这些方法,在细胞水平上分析NABT损伤(其中可以使用组织学检查)仍然存在具有挑战性的问题。我们开发了一种方法,使用与扩散特征函数的二维高斯反卷积相关的拉姆达图分析,将NABT分离为以下解剖结构:1)主要由小神经元和神经胶质组成的结构;2)主要由大神经元组成的结构;3)主要由短轴突组成的结构;4)主要由长轴突组成的结构。对14例传统复发缓解型MS(RRMS)患者和20名年龄匹配的健康志愿者,对每个具有独特扩散特征的分离结构进行DTI衍生参数的统计推断。在所有结构中,RRMS患者的迹值显著更高,分数各向异性值显著更低。此外,主要由短轴突组成的结构的体积分数明显降低,而主要由小神经元和神经胶质组成的结构的体积分数明显增加。这些结果表明,轴突损失和神经胶质增生主要发生在皮质下白质和相邻的深层皮质层,即皮质旁区域。这种使用DTI对NABT损伤进行的以细胞为导向的分析在体内证实了组织学观察结果,即皮质旁区域是MS中最易受损的部位。

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