筛选影响线虫中活性区形成的遗传基因座。

Screening for genetic loci affecting the active zone formation in C. elegans.

机构信息

Department of Genetics and Developmental Biology, Southeast University, Nanjing 210009, China; Ministry of Education Key Laboratory of Developmental Genes and Human Disease, Nanjing 210009, China; E-mail:

出版信息

Neurosci Bull. 2006 Sep;22(5):301-4.

PMID:17690731

Abstract

Objective To screen and identify genetic loci affecting the active zone formation in C. elegans. Methods A SYD-2::GFP reporter was constructed and used as an active zone marker for forward genetic screen to identify genetic loci affecting the active zone formation. Results Eight isolated mutant alleles were characterized from 15,000 haploid genomes. The SYD-2::GFP phenotypes of these mutants are mainly reflected as the changes of number, morphology, distribution of puncta and the gaps appearance. Some mutants also exhibit visible behavioral or physical phenotypes, and aldicarb resistant or sensitive phenotypes. Conclusion These mutants provide the opportunity for further systematic research on the active zone formation and the neurotransmission.

摘要

目的筛选和鉴定影响线虫活性区形成的遗传基因座。

方法构建 SYD-2::GFP 报告基因，作为活性区标记，进行正向遗传学筛选，以鉴定影响活性区形成的遗传基因座。

结果从 15000 个单倍体基因组中分离出 8 个突变等位基因。这些突变体的 SYD-2::GFP 表型主要反映为斑点数量、形态、分布和间隙的变化。一些突变体还表现出明显的行为或物理表型，以及对氨基甲酸酯类杀虫剂抗性或敏感表型。

结论这些突变体为进一步研究活性区形成和神经传递提供了机会。