FtsQ跨膜片段对细胞分裂位点定位的作用。
Contribution of the FtsQ transmembrane segment to localization to the cell division site.
作者信息
Scheffers Dirk-Jan, Robichon Carine, Haan Gert Jan, den Blaauwen Tanneke, Koningstein Gregory, van Bloois Edwin, Beckwith Jon, Luirink Joen
机构信息
Molecular Microbiology, Institute for Molecular Cell Biology, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands.
出版信息
J Bacteriol. 2007 Oct;189(20):7273-80. doi: 10.1128/JB.00723-07. Epub 2007 Aug 10.
Abstract
The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site.
摘要
大肠杆菌细胞分裂蛋白FtsQ是分裂体的核心组成部分。FtsQ是一种双拓扑膜蛋白，具有一个大的C端周质结构域。在这项工作中，我们研究了将FtsQ锚定在细胞质膜中的跨膜区段（TMS）的作用。构建了一组TMS突变体，并分析了它们互补ftsQ突变体的能力。对FtsQ生物合成所涉及的各个步骤的研究表明，一个突变体（L29/32R；V38P）未能在功能上插入膜中，而另一个突变体（L29/32R）正确组装并与FtsB和FtsL相互作用，但未能有效地定位于细胞分裂位点。我们的结果表明，FtsQ TMS在FtsQ定位于分裂位点中发挥作用。
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3
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