Shen Lanlan, Waterland Robert A
Department of Leukemia, University of Texas MD Anderson Cancer Center, USA.
Curr Opin Clin Nutr Metab Care. 2007 Sep;10(5):576-81. doi: 10.1097/MCO.0b013e3282bf6f43.
To provide guidance for investigators who are new to the field of DNA methylation analysis.
Epigenetics is the study of mitotically heritable alterations in gene expression potential that are not mediated by changes in DNA sequence. Recently, it has become clear that nutrition can affect epigenetic mechanisms, causing long-term changes in gene expression. This review focuses on methods for studying the epigenetic mechanism DNA methylation. Recent advances include improvement in high-throughput methods to obtain quantitative data on locus-specific DNA methylation and development of various approaches to study DNA methylation on a genome-wide scale.
No single method of DNA methylation analysis will be appropriate for every application. By understanding the type of information provided by, and the inherent potential for bias and artifact associated with, each method, investigators can select the method most appropriate for their specific research needs.
为DNA甲基化分析领域的新手研究者提供指导。
表观遗传学是研究基因表达潜能中可通过有丝分裂遗传的改变，且这种改变并非由DNA序列变化介导。最近，营养能够影响表观遗传机制并导致基因表达发生长期变化这一点已变得清晰。本综述聚焦于研究表观遗传机制DNA甲基化的方法。近期进展包括高通量方法的改进，以获取基因座特异性DNA甲基化的定量数据，以及开发了各种在全基因组范围内研究DNA甲基化的方法。
没有一种DNA甲基化分析方法适用于所有应用。通过了解每种方法所提供的信息类型以及与之相关的偏差和假象的内在可能性，研究者可以选择最适合其特定研究需求的方法。
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