Orden Alan Van, Jung Jaemyeong
Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.
Biopolymers. 2008 Jan;89(1):1-16. doi: 10.1002/bip.20826.
This article reviews the application of fluorescence correlation spectroscopy (FCS) and related techniques to the study of nucleic acid hairpin conformational fluctuations in free aqueous solutions. Complimentary results obtained using laser-induced temperature jump spectroscopy, single-molecule fluorescence spectroscopy, optical trapping, and biophysical theory are also discussed. The studies cited reveal that DNA and RNA hairpin folding occurs by way of a complicated reaction mechanism involving long- and short-lived reaction intermediates. Reactions occurring on the subnanoseconds to seconds time scale have been observed, pointing out the need for experimental techniques capable of probing a broad range of reaction times in the study of such complex, multistate reactions.
本文综述了荧光相关光谱法(FCS)及相关技术在研究游离水溶液中核酸发夹构象波动方面的应用。还讨论了使用激光诱导温度跃变光谱法、单分子荧光光谱法、光镊技术和生物物理理论所获得的补充结果。所引用的研究表明,DNA和RNA发夹折叠是通过一种复杂的反应机制发生的,该机制涉及长寿命和短寿命的反应中间体。已观察到在亚纳秒到秒时间尺度上发生的反应,这表明在研究此类复杂的多态反应时,需要能够探测广泛反应时间范围的实验技术。