Furuno N, Nakagawa K, Eguchi U, Ohtsubo M, Nishimoto T, Soeda E
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan.
Genomics. 1991 Oct;11(2):459-61. doi: 10.1016/0888-7543(91)90156-9.
Total genomic DNA of the human RCC1 gene was isolated from HeLa DNA and its complete nucleotide sequence (34,641 bp) was determined by the shotgun sequencing method. The exon-intron junctions were precisely assigned to this sequence by comparing the nucleotide sequence of RCC1 genomic DNA with that of its cDNA. The RCC1 gene was found to have 14 exons, 8 of which (starting from the seventh one) coded the seven repeated sequences of RCC1 protein. A single exon corresponded roughly to each repeat of the RCC1 protein except for the middle one, indicating that the RCC1 gene was generated through amplification of a primordial exon. Primer extension analysis revealed the presence of an internal promoter.
人RCC1基因的全基因组DNA从HeLa细胞DNA中分离得到，其完整的核苷酸序列（34,641 bp）通过鸟枪法测序法测定。通过将RCC1基因组DNA的核苷酸序列与其cDNA的序列进行比较，外显子-内含子连接位点被精确地定位到该序列上。发现RCC1基因有14个外显子，其中8个（从第7个开始）编码RCC1蛋白的7个重复序列。除中间的重复序列外，单个外显子大致对应RCC1蛋白的每个重复序列，这表明RCC1基因是通过原始外显子的扩增产生的。引物延伸分析揭示了一个内部启动子的存在。
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