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膳食抗氧化剂作为血红素诱导的亚油酸过氧化反应的抑制剂:作用机制与协同作用

Dietary antioxidants as inhibitors of the heme-induced peroxidation of linoleic acid: mechanism of action and synergism.

作者信息

Goupy Pascale, Vulcain Emmanuelle, Caris-Veyrat Catherine, Dangles Olivier

机构信息

UMR408 Sécurité et Qualité des Produits d'Origine Végétale, INRA, Université d'Avignon, Domaine St Paul, Site Agroparc, 84914 Avignon, France.

出版信息

Free Radic Biol Med. 2007 Sep 15;43(6):933-46. doi: 10.1016/j.freeradbiomed.2007.06.013. Epub 2007 Jun 16.

Abstract

In this work, a quantitative kinetic model for investigating the heme-induced peroxidation of linoleic acid and its inhibition by two important dietary antioxidants, quercetin and alpha-tocopherol, is developed. The main conclusions of this work are: (1) The time dependence of the lipid hydroperoxide concentration is critically dependent on the rate constant for lipid hydroperoxide cleavage, initial fraction of lipid hydroperoxides among the pool of conjugated dienes, and rate of heme degradation. (2) The lipophilic antioxidant alpha-tocopherol acts as a chain-breaking antioxidant that quickly reduces 1-2 eq of lipid peroxyl radicals (inhibition of propagation), whereas the more hydrophilic antioxidant quercetin is only marginally chain-breaking but capable of reducing 4-5 eq of iron-oxo initiator (inhibition of initiation). (3) Based on comparisons between experimental peroxidation curves and simulated curves assuming additivity, it can be concluded that combinations of alpha-tocopherol and quercetin are generally synergistic. The kinetic analysis and HPLC titrations of the antioxidants both suggest that synergism mainly arises from a capacity of alpha-tocopherol to regenerate some quercetin oxidation products still endowed with a reducing activity.

摘要

在本研究中,建立了一个定量动力学模型,用于研究血红素诱导的亚油酸过氧化反应以及两种重要的膳食抗氧化剂槲皮素和α-生育酚对该反应的抑制作用。本研究的主要结论如下:(1)脂质氢过氧化物浓度的时间依赖性主要取决于脂质氢过氧化物裂解的速率常数、共轭二烯池中脂质氢过氧化物的初始比例以及血红素降解速率。(2)亲脂性抗氧化剂α-生育酚作为链断裂抗氧化剂,能迅速还原1-2当量的脂质过氧自由基(抑制链增长),而亲水性更强的抗氧化剂槲皮素仅具有微弱的链断裂能力,但能够还原4-5当量的铁氧引发剂(抑制引发)。(3)基于实验过氧化曲线与假设具有加和性的模拟曲线之间的比较,可以得出结论,α-生育酚和槲皮素的组合通常具有协同作用。抗氧化剂的动力学分析和HPLC滴定均表明,协同作用主要源于α-生育酚能够再生一些仍具有还原活性的槲皮素氧化产物。

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