Bojesen Anders M, Larsen Jesper, Pedersen Anders G, Mörner Torsten, Mattson Roland, Bisgaard Magne
Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark.
J Wildl Dis. 2007 Jul;43(3):345-52. doi: 10.7589/0090-3558-43.3.345.
Eight atypical Mannheimia isolates were isolated from lesions in roe deer (Capreolus capreolus). Traditional classification based on morphologic and physiologic traits showed that they belong to a distinct biogroup (taxon) within genus Mannheimia. Extensive phenotypic characterization suggested that the isolates should be classified as M. granulomatis, although the presence of distinct traits justified their classification into a separate biogroup within this species. Phylogenetic analyses based on 16S rRNA sequences from two roe deer isolates and 41 other Mannheimia strains supported that the roe deer isolates form a monophyletic group within M. granulomatis. The lktA genotype was present in all roe deer isolates based on Southern blot analysis, whereas the corresponding beta-hemolytic phenotype was absent in one of these isolates.
从狍（Capreolus capreolus）的病变部位分离出8株非典型曼氏杆菌菌株。基于形态学和生理学特征的传统分类表明，它们属于曼氏杆菌属内一个独特的生物群（分类单元）。广泛的表型特征表明，这些分离株应归类为肉芽肿曼氏杆菌，尽管存在明显特征证明它们可归入该物种内的一个单独生物群。基于来自两株狍分离株和其他41株曼氏杆菌菌株的16S rRNA序列的系统发育分析支持，狍分离株在肉芽肿曼氏杆菌内形成一个单系群。基于Southern印迹分析，所有狍分离株均存在lktA基因型，而其中一株分离株不存在相应的β-溶血表型。
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