Casado-Terrones Silvia, Segura-Carretero Antonio, Busi Simone, Dinelli Giovanni, Fernández-Gutiérrez Alberto
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain.
Electrophoresis. 2007 Aug;28(16):2882-7. doi: 10.1002/elps.200600614.
We have developed a sensitive CE method to determine eight tetracyclines (TCs) (chlortetracycline, demeclocycline, doxycycline, methacycline, minocycline, oxytetracycline, TC, and rolitetracycline (RTC)) in honey samples. The running buffer was 150 mM sodium borate (pH 9.8) and 2.5% 2-propanol with 15 s hydrodynamic injection at 25 kV. We have also developed an SPE procedure with a C18 cartridge as a clean-up step. Analytes were detected at 360 nm in less than 16 min. LODs ranged in honey from 23.9 microg/kg for TC to 49.3 microg/kg for RTC. Seven samples of Spanish honey of different floral origins were examined. None of them showed contamination with these antibiotics using the proposed method.
我们开发了一种灵敏的毛细管电泳（CE）方法，用于测定蜂蜜样品中的八种四环素（TCs）（金霉素、地美环素、强力霉素、甲烯土霉素、米诺环素、土霉素、四环素（TC）和罗利环素（RTC））。运行缓冲液为150 mM硼酸钠（pH 9.8）和2.5%异丙醇，在25 kV下进行15 s的 hydrodynamic 进样。我们还开发了一种以C18柱为净化步骤的固相萃取（SPE）程序。在不到16分钟的时间内，在360 nm处检测分析物。蜂蜜中的检测限范围为：TC为23.9微克/千克，RTC为49.3微克/千克。对七个不同花卉来源的西班牙蜂蜜样品进行了检测。使用所提出的方法，这些样品均未显示受到这些抗生素的污染。
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