通过自旋标记侧链的电子顺磁共振光谱描述蛋白质细丝的结构和组装。
Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains.
作者信息
Budamagunta Madhu, Hess John, Fitzgerald Paul, Voss John
机构信息
Department of Biochemistry & Molecular Medicine, University of California Davis, Davis, CA 95616, USA.
出版信息
Cell Biochem Biophys. 2007;48(1):45-53. doi: 10.1007/s12013-007-0035-4.
In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.
在本综述中，我们总结了通过对定点自旋标记进行电子顺磁共振（EPR）光谱研究中间丝（IF）结构和组装的方法。我们以波形蛋白（一种同聚III型IF蛋白）为例，证明了该方法是研究蛋白质丝结构和组装的通用范式。这些策略将有助于探索其他丝状或易于聚集的系统的结构和组装特性。
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