Suppr超能文献

与疾病暴发相关的肺炎链球菌血清型中存在非溶血性肺炎溶血素。

Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks.

作者信息

Jefferies Johanna M C, Johnston Calum H G, Kirkham Lea-Ann S, Cowan Graeme J M, Ross Kirsty S, Smith Andrew, Clarke Stuart C, Brueggemann Angela B, George Robert C, Pichon Bruno, Pluschke Gerd, Pfluger Valentin, Mitchell Tim J

机构信息

Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow, United Kingdom.

出版信息

J Infect Dis. 2007 Sep 15;196(6):936-44. doi: 10.1086/520091. Epub 2007 Aug 6.

Abstract

Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.

摘要

肺炎溶血素是人类病原体肺炎链球菌的一种重要毒力因子。对121株临床分离的肺炎链球菌的ply基因进行序列分析,发现了多个等位基因。选择22株菌株进行进一步分析,发现了14个蛋白质等位基因。其中5个先前已有报道,其余9个是新发现的。使用细胞裂解物来测定肺炎溶血素蛋白的特异性溶血活性。6株菌株无溶血活性,其余16株有不同程度的溶血活性。我们报告,先前在血清型1、序列型(ST)306分离株中报道的非溶血等位基因,也存在于属于ST53谱系的多个血清型8肺炎球菌分离株中。已知血清型1和8肺炎球菌与侵袭性疾病暴发有关。因此,非溶血肺炎溶血素等位基因与肺炎链球菌暴发相关血清型的优势克隆有关。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验